Hydrogen peroxide enhances phagocytic activity of ameboid microglia

Hidetaka Takeda, Minoru Tomita, Norio Tanahashi, Masahiro Kobari, Masako Yokoyama, Masaki Takao, Daisuke Ito, Yasuo Fukuuchi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Microglia are considered to serve as a guardian of the brain. In achieving this task, they have been observed to transform into a reactive form and then an ameboid form. Several substances are implicated in the control of such behavior. We examined the effect of hydrogen peroxide on cultured microglia of ameboid form obtained from the fetal rat brain employing video-enhanced contrast-differential interference contrast microscopy. Microglia harvested from the culture bottle were observed to float in a spherical shape with abundant filopodia on the surface. However, on coming into contact with the glass surface of a cover slip, they immediately transformed into an ameboid form. The microglia spread themselves out, surrounded by thin transparent lammellipodia, which would not be clearly observable by either light microscopy or electron microscopy. In a concentration-dependent fashion, 10-3-10-1 M hydrogen peroxide solution enhanced the ruffling process of the lamellipodia and formation of vesicles (phagosomes), which displayed a typical phagocytotic form. It is concluded that an increase in free radicals in ischemic tissue tends to facilitate the phagocytosis of ameboid microglia as macrophages.

Original languageEnglish
Pages (from-to)5-8
Number of pages4
JournalNeuroscience Letters
Volume240
Issue number1
DOIs
Publication statusPublished - 1998 Jan 2

Fingerprint

Microglia
Hydrogen Peroxide
Pseudopodia
Interference Microscopy
Phagosomes
Behavior Control
Brain
Phagocytosis
Free Radicals
Glass
Microscopy
Electron Microscopy
Macrophages
Light

Keywords

  • Filopodia
  • Lamellipodia
  • Microglia
  • Phagosome
  • Ruffling
  • Vacuolization
  • Video- enhanced contrast-differential interference contrast microscopy

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Takeda, H., Tomita, M., Tanahashi, N., Kobari, M., Yokoyama, M., Takao, M., ... Fukuuchi, Y. (1998). Hydrogen peroxide enhances phagocytic activity of ameboid microglia. Neuroscience Letters, 240(1), 5-8. https://doi.org/10.1016/S0304-3940(97)00917-8

Hydrogen peroxide enhances phagocytic activity of ameboid microglia. / Takeda, Hidetaka; Tomita, Minoru; Tanahashi, Norio; Kobari, Masahiro; Yokoyama, Masako; Takao, Masaki; Ito, Daisuke; Fukuuchi, Yasuo.

In: Neuroscience Letters, Vol. 240, No. 1, 02.01.1998, p. 5-8.

Research output: Contribution to journalArticle

Takeda, H, Tomita, M, Tanahashi, N, Kobari, M, Yokoyama, M, Takao, M, Ito, D & Fukuuchi, Y 1998, 'Hydrogen peroxide enhances phagocytic activity of ameboid microglia', Neuroscience Letters, vol. 240, no. 1, pp. 5-8. https://doi.org/10.1016/S0304-3940(97)00917-8
Takeda H, Tomita M, Tanahashi N, Kobari M, Yokoyama M, Takao M et al. Hydrogen peroxide enhances phagocytic activity of ameboid microglia. Neuroscience Letters. 1998 Jan 2;240(1):5-8. https://doi.org/10.1016/S0304-3940(97)00917-8
Takeda, Hidetaka ; Tomita, Minoru ; Tanahashi, Norio ; Kobari, Masahiro ; Yokoyama, Masako ; Takao, Masaki ; Ito, Daisuke ; Fukuuchi, Yasuo. / Hydrogen peroxide enhances phagocytic activity of ameboid microglia. In: Neuroscience Letters. 1998 ; Vol. 240, No. 1. pp. 5-8.
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