Hydrogen sulfide as an endogenous modulator of biliary bicarbonate excretion in the rat liver

Kimihito Fujii, Tadayuki Sakuragawa, Misato Kashiba, Yasoo Sugiura, Mieko Kondo, Kayo Maruyama, Nobuhito Goda, Yuji Nimura, Makoto Suematsu

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20 Citations (Scopus)

Abstract

Cystathionine γ-lyase (CSE) is an enzyme catalyzing cystathionine and cysteine to yield cysteine and hydrogen sulfide (H2S), respectively. This study aimed to examine if H2S generated from the enzyme could serve as an endogenous regulator of hepatobiliary function. Gas Chromatographic analyses indicated that, among rat organs herein examined, liver constituted one of the greatest components of H2S generation in the body, at 100 μmol/g of tissue, comparable to that in kidney and 1.5-fold greater than that in brain, where roles of the gas in the regulation of neurotransmission were reported previously. At least half of the gas amount in the liver appeared to be derived from CSE, because blockade of the enzyme by propargylglycine suppressed it by 50%. Immunohistochemistry revealed that CSE occurs not only in hepatocytes, but also in bile duct. In livers in vivo, as well as in those perfused ex vivo, treatment with the CSE inhibitor induced choleresis by stimulating the basal excretion of bicarbonate in bile samples. Transportal supplementation of NaHS at 30 μmol/L, but not that of N-acetylcysteine as a cysteine donor, abolished these changes elicited by the CSE inhibitor in the perfused liver. The changes elicited by the CSE blockade did not coincide with alterations in hepatic vascular resistance, showing little involvement of vasodilatory effects of the gas in these events, if any. These results first provided evidence that H2S generated through CSE modulates biliary bicarbonate excretion and is thus a determinant of bile salt-independent bile formation in the rat liver.

Original languageEnglish
Pages (from-to)788-794
Number of pages7
JournalAntioxidants and Redox Signaling
Volume7
Issue number5-6
DOIs
Publication statusPublished - 2005 May

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Cystathionine
Hydrogen Sulfide
Lyases
Bicarbonates
Liver
Modulators
Rats
Gases
Cysteine
Bile
Enzymes
Hepatobiliary Elimination
Acetylcysteine
Bile Ducts
Bile Acids and Salts
Synaptic Transmission
Vascular Resistance
Gas Chromatography
Ducts
Hepatocytes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Fujii, K., Sakuragawa, T., Kashiba, M., Sugiura, Y., Kondo, M., Maruyama, K., ... Suematsu, M. (2005). Hydrogen sulfide as an endogenous modulator of biliary bicarbonate excretion in the rat liver. Antioxidants and Redox Signaling, 7(5-6), 788-794. https://doi.org/10.1089/ars.2005.7.788

Hydrogen sulfide as an endogenous modulator of biliary bicarbonate excretion in the rat liver. / Fujii, Kimihito; Sakuragawa, Tadayuki; Kashiba, Misato; Sugiura, Yasoo; Kondo, Mieko; Maruyama, Kayo; Goda, Nobuhito; Nimura, Yuji; Suematsu, Makoto.

In: Antioxidants and Redox Signaling, Vol. 7, No. 5-6, 05.2005, p. 788-794.

Research output: Contribution to journalArticle

Fujii, K, Sakuragawa, T, Kashiba, M, Sugiura, Y, Kondo, M, Maruyama, K, Goda, N, Nimura, Y & Suematsu, M 2005, 'Hydrogen sulfide as an endogenous modulator of biliary bicarbonate excretion in the rat liver', Antioxidants and Redox Signaling, vol. 7, no. 5-6, pp. 788-794. https://doi.org/10.1089/ars.2005.7.788
Fujii, Kimihito ; Sakuragawa, Tadayuki ; Kashiba, Misato ; Sugiura, Yasoo ; Kondo, Mieko ; Maruyama, Kayo ; Goda, Nobuhito ; Nimura, Yuji ; Suematsu, Makoto. / Hydrogen sulfide as an endogenous modulator of biliary bicarbonate excretion in the rat liver. In: Antioxidants and Redox Signaling. 2005 ; Vol. 7, No. 5-6. pp. 788-794.
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