Hydrophobized thermoresponsive copolymer brushes for cell separation by multistep temperature change

Kenichi Nagase, Yuri Hatakeyama, Tatsuya Shimizu, Katsuhisa Matsuura, Masayuki Yamato, Naoya Takeda, Teruo Okano

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

For preparing a thermally modulated biointerface that separates cells without the modification of cell surfaces for regenerative medicine and tissue engineering, poly(N-isopropylacrylamide-co-butyl methacrylate) (P(IPAAm-co-BMA), thermo-responsive hydrophobic copolymer brushes with various BMA composition were formed on glass substrate through a surface-initiated atom transfer radical polymerization (ATRP). Characterization of the prepared surface was performed by X-ray photoelectron spectroscopy (XPS), attenuated total reflection Fourier transform infrared spectroscopy (ATR/FT-IR), and gel-permeation chromatography (GPC) measurement. Prepared copolymer brush surfaces were characterized by observing the adhesion (37 C) and detachment (20 or 10 C) of four types of human cells: human umbilical vein endothelial cells (HUVECs), normal human dermal fibroblasts (NHDFs), human aortic smooth muscle cells (SMCs), and human skeletal muscle myoblast cells (HSMMs). HUVECs and NHDFs exhibited their effective detachment temperature at 20 and 10 C, respectively. Using cells' intrinsic temperature sensitivity for detachment from the copolymer brush, a mixture of green fluorescent protein (GFP)-expressing HUVECs (GFP-HUVECs) and NHDFs was separated.

Original languageEnglish
Pages (from-to)3423-3433
Number of pages11
JournalBiomacromolecules
Volume14
Issue number10
DOIs
Publication statusPublished - 2013 Oct 14
Externally publishedYes

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Brushes
Endothelial cells
Fibroblasts
Copolymers
Muscle
Cells
Temperature
Atom transfer radical polymerization
Gel permeation chromatography
Green Fluorescent Proteins
Tissue engineering
Fourier transform infrared spectroscopy
Adhesion
X ray photoelectron spectroscopy
Proteins
Glass
Substrates
Chemical analysis

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Nagase, K., Hatakeyama, Y., Shimizu, T., Matsuura, K., Yamato, M., Takeda, N., & Okano, T. (2013). Hydrophobized thermoresponsive copolymer brushes for cell separation by multistep temperature change. Biomacromolecules, 14(10), 3423-3433. https://doi.org/10.1021/bm4006722

Hydrophobized thermoresponsive copolymer brushes for cell separation by multistep temperature change. / Nagase, Kenichi; Hatakeyama, Yuri; Shimizu, Tatsuya; Matsuura, Katsuhisa; Yamato, Masayuki; Takeda, Naoya; Okano, Teruo.

In: Biomacromolecules, Vol. 14, No. 10, 14.10.2013, p. 3423-3433.

Research output: Contribution to journalArticle

Nagase, K, Hatakeyama, Y, Shimizu, T, Matsuura, K, Yamato, M, Takeda, N & Okano, T 2013, 'Hydrophobized thermoresponsive copolymer brushes for cell separation by multistep temperature change', Biomacromolecules, vol. 14, no. 10, pp. 3423-3433. https://doi.org/10.1021/bm4006722
Nagase, Kenichi ; Hatakeyama, Yuri ; Shimizu, Tatsuya ; Matsuura, Katsuhisa ; Yamato, Masayuki ; Takeda, Naoya ; Okano, Teruo. / Hydrophobized thermoresponsive copolymer brushes for cell separation by multistep temperature change. In: Biomacromolecules. 2013 ; Vol. 14, No. 10. pp. 3423-3433.
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