Hydrostatic pressure modulates mRNA expressions for matrix proteins in human meniscal cells

Toru Suzuki, Takashi Toyoda, Hiroshi Suzuki, Noriyuki Hisamori, Hideo Matsumoto, Yoshiaki Toyama

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

There have been few reports describing the effects of mechanical loading on the metabolism of meniscal cells. The aim of this study was to investigate the effects of hydrostatic pressure on meniscal cell metabolism. Human meniscal cells were cultured in alginate beads for 3 days. They were then subjected to 4 MPa hydrostatic pressure for 4 hours in either a static or cyclic (1 Hz) mode using a specially designed and constructed system. Immediately after the pressure application, the messenger RNA levels for aggrecan, type I collagen, matrix metalloproteinases (MMP) -1, -3, -9, -13 and tissue inhibitors of metalloproteinases (TIMP) -1 and -2 were measured. It was found that the application of static hydrostatic pressure caused a significant decrease in mRNA expression for MMP-1 and -13 (p < 0.05). In contrast, the application of cyclic hydrostatic pressure was associated with a significant increase in type I collagen (p < 0.01), TIMP-1 and -2 mRNA expression (p < 0.01). These results would suggest that hydrostatic pressure in isolation can modulate mRNA expressions for matrix proteins in meniscal cells.

Original languageEnglish
Pages (from-to)611-622
Number of pages12
JournalBiorheology
Volume43
Issue number5
Publication statusPublished - 2006

Fingerprint

Hydrostatic Pressure
Messenger RNA
Tissue Inhibitor of Metalloproteinase-2
Matrix Metalloproteinase 1
Tissue Inhibitor of Metalloproteinase-1
Collagen Type I
Proteins
Matrix Metalloproteinase 13
Aggrecans
Matrix Metalloproteinase 3
Cultured Cells
Pressure

Keywords

  • Extracellular matrix
  • Hydrostatic pressure
  • Mechanical stress
  • Meniscal cells
  • mRNA expression

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Suzuki, T., Toyoda, T., Suzuki, H., Hisamori, N., Matsumoto, H., & Toyama, Y. (2006). Hydrostatic pressure modulates mRNA expressions for matrix proteins in human meniscal cells. Biorheology, 43(5), 611-622.

Hydrostatic pressure modulates mRNA expressions for matrix proteins in human meniscal cells. / Suzuki, Toru; Toyoda, Takashi; Suzuki, Hiroshi; Hisamori, Noriyuki; Matsumoto, Hideo; Toyama, Yoshiaki.

In: Biorheology, Vol. 43, No. 5, 2006, p. 611-622.

Research output: Contribution to journalArticle

Suzuki, T, Toyoda, T, Suzuki, H, Hisamori, N, Matsumoto, H & Toyama, Y 2006, 'Hydrostatic pressure modulates mRNA expressions for matrix proteins in human meniscal cells', Biorheology, vol. 43, no. 5, pp. 611-622.
Suzuki T, Toyoda T, Suzuki H, Hisamori N, Matsumoto H, Toyama Y. Hydrostatic pressure modulates mRNA expressions for matrix proteins in human meniscal cells. Biorheology. 2006;43(5):611-622.
Suzuki, Toru ; Toyoda, Takashi ; Suzuki, Hiroshi ; Hisamori, Noriyuki ; Matsumoto, Hideo ; Toyama, Yoshiaki. / Hydrostatic pressure modulates mRNA expressions for matrix proteins in human meniscal cells. In: Biorheology. 2006 ; Vol. 43, No. 5. pp. 611-622.
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