Hydroxylated and non-hydroxylated sulfatide are distinctly distributed in the human cerebral cortex

D. Yuki, Yuki Sugiura, N. Zaima, H. Akatsu, Y. Hashizume, T. Yamamoto, M. Fujiwara, K. Sugiyama, M. Setou

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Sulfatide (ST) is a sphingolipid with an important role in the central nervous system as a major component of the myelin sheath. ST contains a structurally variable ceramide moiety, with a fatty acid substituent of varying carbon-chain length and double-bond number. Hydroxylation at the α-2 carbon position of the fatty acid is found in half the population of ST molecules. Recent genetic studies of fatty acid 2-hydroxylase (FA2H) indicate that these hydroxylated sphingolipids influence myelin sheath stability. However, their distribution is unknown. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) enables the analysis of distinct distributions of individual ST molecular species in tissue section. We examined human cerebral cortex tissue sections with MALDI-IMS, identifying and characterizing the distributions of 14 ST species. The distribution analysis reveals that the composition ratios of non-hydroxylated/hydroxylated STs are clearly reversed at the border between white and gray matter; the hydroxylated group is the dominant ST species in the gray matter. These results suggest that hydroxylated STs are highly expressed in oligodendrocytes in gray matter and might form stable myelin sheaths. As a clinical application, we analyzed a brain with Alzheimer's disease (AD) as a representative neurodegenerative disease. Although previous studies of AD pathology have reported that the amount of total ST is decreased in the cerebral cortex, as far as the compositional distributions of STs are concerned, AD brains were similar to those in control brains. In conclusion, we suggest that MALDI-IMS is a useful tool for analysis of the distributions of various STs and this application might provide novel insight in the clinical study of demyelinating diseases.

Original languageEnglish
Pages (from-to)44-53
Number of pages10
JournalNeuroscience
Volume193
DOIs
Publication statusPublished - 2011 Oct 13
Externally publishedYes

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Sulfoglycosphingolipids
Cerebral Cortex
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Myelin Sheath
Alzheimer Disease
Sphingolipids
Fatty Acids
Brain
Carbon
Ceramides
Oligodendroglia
Demyelinating Diseases
Hydroxylation
Mixed Function Oxygenases
Neurodegenerative Diseases
Central Nervous System
Pathology

Keywords

  • Alzheimer's disease
  • Cerebral cortex
  • Hydroxylation
  • Imaging mass spectrometry
  • Myelin sheath
  • Sulfatide

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hydroxylated and non-hydroxylated sulfatide are distinctly distributed in the human cerebral cortex. / Yuki, D.; Sugiura, Yuki; Zaima, N.; Akatsu, H.; Hashizume, Y.; Yamamoto, T.; Fujiwara, M.; Sugiyama, K.; Setou, M.

In: Neuroscience, Vol. 193, 13.10.2011, p. 44-53.

Research output: Contribution to journalArticle

Yuki, D, Sugiura, Y, Zaima, N, Akatsu, H, Hashizume, Y, Yamamoto, T, Fujiwara, M, Sugiyama, K & Setou, M 2011, 'Hydroxylated and non-hydroxylated sulfatide are distinctly distributed in the human cerebral cortex', Neuroscience, vol. 193, pp. 44-53. https://doi.org/10.1016/j.neuroscience.2011.07.045
Yuki, D. ; Sugiura, Yuki ; Zaima, N. ; Akatsu, H. ; Hashizume, Y. ; Yamamoto, T. ; Fujiwara, M. ; Sugiyama, K. ; Setou, M. / Hydroxylated and non-hydroxylated sulfatide are distinctly distributed in the human cerebral cortex. In: Neuroscience. 2011 ; Vol. 193. pp. 44-53.
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