Hypercapnic acidosis attenuates endotoxin-induced nuclear factor-κB activation

Kei Takeshita, Yukio Suzuki, Kazumi Nishio, Osamu Takeuchi, Kyoko Toda, Hiroyasu Kudo, Naoki Miyao, Makoto Ishii, Nagato Sato, Katsuhiko Naoki, Takuya Aoki, Koichi Suzuki, Rika Hiraoka, Kazuhiro Yamaguchi

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116 Citations (Scopus)

Abstract

Although permissive hypercapnia improves the prognosis of patients with acute respiratory distress syndrome, it has not been conclusively determined whether hypercapnic acidosis (HA) is harmful or beneficial to sustained inflammation of the lung. The present study was designed to explore the molecular mechanism of HA in modifying lipopolysaccharide (LPS)-associated signals in pulmonary endothelial cells. LPS elicited degradation of inhibitory protein κB (IκB)-α, but not IκB-β, resulting in activation of nuclear factor (NF)-κB in human pulmonary artery endothelial cells. Exposure to HA significantly attenuated LPS-induced NF-κB activation through suppressing IκB-α degradation. Isocapnic acidosis and buffered hypercapnia showed qualitatively similar but quantitatively smaller effects. HA did not attenuate the LPS-enhanced activation of activator protein-1. Following the reduced NF-κB activation, HA suppressed the mRNA and protein levels of intercellular adhesion molecule-1 and interleukin-8, resulting in a decrease in both lactate dehydrogenase release into the medium and neutrophil adherence to LPS-activated human pulmonary artery endothelial cells. In contrast, HA did not inhibit LPS-enhanced neutrophil expression of integrin, Mac-1. Based on these findings, we concluded that hypercapnic acidosis would have anti-inflammatory effects essentially through a mechanism inhibiting NF-κB activation, leading to downregulation of intercellular adhesion molecule-1 and interleukin-8, which in turn inhibits neutrophil adherence to pulmonary endothelial cells.

Original languageEnglish
Pages (from-to)124-132
Number of pages9
JournalAmerican journal of respiratory cell and molecular biology
Volume29
Issue number1
DOIs
Publication statusPublished - 2003 Jul 1

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ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Takeshita, K., Suzuki, Y., Nishio, K., Takeuchi, O., Toda, K., Kudo, H., Miyao, N., Ishii, M., Sato, N., Naoki, K., Aoki, T., Suzuki, K., Hiraoka, R., & Yamaguchi, K. (2003). Hypercapnic acidosis attenuates endotoxin-induced nuclear factor-κB activation. American journal of respiratory cell and molecular biology, 29(1), 124-132. https://doi.org/10.1165/rcmb.2002-0126OC