Hyperexpression of inducible costimulator on lamina propria mononuclear cells in rat dextran sulfate sodium colitis

Ken Ishii, Takanori Kanai, Teruji Totsuka, Koji Uraushihara, Takahiro Ishikura, Motomi Yamazaki, Ryoici Okamoto, Akihiro Araki, Tatsuya Miyata, Katsuaki Tezuka, Tetsuya Nakamura, Mamoru Watanabe

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Background and Aims: The authors have previously shown that a third member of the CD28 family, inducible costimulator (ICOS), was increased in the inflamed intestinal mucosa of murine experimental colitis, and that the blockade of ICOS ameliorated the development of colitis. However, the role of ICOS in rat intestinal inflammation and its expression profile remains unclear. In the present study, the authors investigated the involvement of ICOS in the development of rat dextran sulfate sodium (DSS)-induced colitis, and the therapeutic potential of anti-ICOS monoclonal antibody (mAb) in colitis. Methods: The authors first examined expression of ICOS protein in normal rat by immunohistochemistry and flow cytometry. Sprague-Dawley rats were fed 3.0% DSS. The expression of ICOS on infiltrating lamina propria mononuclear cells and splenocytes were examined. The DSS-fed rats were then administered anti-ICOS mAb to test its effect on the development of colitis. Results: Unlike mice and human, ICOS was expressed on a part of CD4+ T-cells from the thymus, spleen, mesenteric lymph nodes and lamina propria. Levels of ICOS on CD4 + T-cells from the spleen and colonic lamina propria were significantly upregulated after Concanavalin A (Con A) stimulation. In addition, ICOS was also upregulated on CD4+ T-cells from DSS-fed rats compared with those from non DSS-fed rats. However, anti-ICOS mAb did not ameliorate the development of both acute and chronic DSS colitis. Conclusion: These results suggest that the different expression of ICOS in rats plays a distinct role in rat intestinal inflammation.

Original languageEnglish
Pages (from-to)174-181
Number of pages8
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume19
Issue number2
DOIs
Publication statusPublished - 2004 Feb
Externally publishedYes

Keywords

  • CD28
  • Costimulation
  • Crohn's disease
  • Inducible costimulator
  • Ulcerative colitis

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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