TY - JOUR
T1 - Hypertrophic stimuli augment expression of cMG1/ERF-1, a putative zinc- finger motif transcription factor, in rat cardiomyocytes
AU - Manabe, Tomohiro
AU - Fukuda, Keiichi
AU - Pan, Jing
AU - Nagasaki, Koichi
AU - Yamaguchi, Ken
AU - Ogawa, Satoshi
N1 - Funding Information:
This study was supported by research grants of ‘Research for the Future’ Program from the Japan Society for the Promotion of Science (JSPS-RFTF97I00201), and research grants from the Ministry of Education, Science and Culture, Japan, the Health Science Research Grants for Advanced Medical Technology from Ministry of Welfare, Japan.
PY - 1999/12/10
Y1 - 1999/12/10
N2 - We isolated the gene for cMG1/ERF-1, a known putative zinc-finger transcription factor, by differential display of mRNA extracted from cardiomyocytes with and without leukemia inhibitory factor (LIF) stimulation. LIF induced cMG1/ERF-1 mRNA at 15 min, and levels peaked at 10-fold initial levels at 30 min. cMG1/ERF-1 expression was inhibited by AG490 (JAK2 inhibitor) and genistein, but was unaffected by PD98059 or wortmannin. Phenylephrine, angiotensin II and endothelin-1 also induced cMG1/ERF-1 expression. Mechanical stretch in vitro and acute pressure overload in vivo increased cMG1/ERF-1 expression. To our knowledge, this is the first report showing that the cMG1/ERF-1 gene can be induced by various hypertrophic stimuli, and that Janus kinase 2 is involved in this process.
AB - We isolated the gene for cMG1/ERF-1, a known putative zinc-finger transcription factor, by differential display of mRNA extracted from cardiomyocytes with and without leukemia inhibitory factor (LIF) stimulation. LIF induced cMG1/ERF-1 mRNA at 15 min, and levels peaked at 10-fold initial levels at 30 min. cMG1/ERF-1 expression was inhibited by AG490 (JAK2 inhibitor) and genistein, but was unaffected by PD98059 or wortmannin. Phenylephrine, angiotensin II and endothelin-1 also induced cMG1/ERF-1 expression. Mechanical stretch in vitro and acute pressure overload in vivo increased cMG1/ERF-1 expression. To our knowledge, this is the first report showing that the cMG1/ERF-1 gene can be induced by various hypertrophic stimuli, and that Janus kinase 2 is involved in this process.
KW - Cardiac hypertrophy
KW - Leukemia inhibitory factor
KW - Rat cardiomyocyte
KW - cMG1/ERF-1
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U2 - 10.1016/S0014-5793(99)01601-4
DO - 10.1016/S0014-5793(99)01601-4
M3 - Article
C2 - 10601634
AN - SCOPUS:0033405878
SN - 0014-5793
VL - 463
SP - 39
EP - 42
JO - FEBS Letters
JF - FEBS Letters
IS - 1-2
ER -