Hypoxia-induced cerebral angiogenesis in mouse cortex with two-photon microscopy

Kazuto Masamoto, Hiroyuki Takuwa, Yutaka Tomita, Haruki Toriumi, Miyuki Unekawa, Junko Taniguchi, Hiroshi Kawaguchi, Yoshiaki Itoh, Norihiro Suzuki, Hiroshi Ito, Iwao Kanno

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

To better understand cellular interactions of the cerebral angiogenesis induced by hypoxia, a spatiotemporal dynamics of cortical microvascular restructuring during an exposure to continuous hypoxia was characterized with in vivo two-photon microscopy in mouse cortex. The mice were prepared with a closed cranial window over the sensory-motor cortex and housed in 8-9 % oxygen room for 2-4 weeks. Before beginning the hypoxic exposure, two-photon imaging of cortical microvasculature was performed, and the follow-up imaging was conducted weekly in the identical locations. We observed that 1-2 weeks after the onset of hypoxic exposure, a sprouting of new vessels appeared from the existing capillaries. An average emergence rate of the new vessel was 15 vessels per unit volume (mm3). The highest emergence rate was found in the cortical depths of 100-200 μm, indicating no spatial uniformity among the cortical layers. Further, a leakage of fluorescent dye (sulforhodamine 101) injected into the bloodstream was not detected, suggesting that the blood-brain barrier (BBB) was maintained. Future studies are needed to elucidate the roles of perivascular cells (e.g., pericyte, microglia, and astroglia) in a process of this hypoxia-induced angiogenesis, such as sprouting, growth, and merger with the existing capillary networks, while maintaining the BBB.

Original languageEnglish
Title of host publicationOxygen Transport to Tissue XXXV
PublisherSpringer New York LLC
Pages15-20
Number of pages6
ISBN (Print)9781461472568
DOIs
Publication statusPublished - 2013

Publication series

NameAdvances in Experimental Medicine and Biology
Volume789
ISSN (Print)0065-2598

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Masamoto, K., Takuwa, H., Tomita, Y., Toriumi, H., Unekawa, M., Taniguchi, J., Kawaguchi, H., Itoh, Y., Suzuki, N., Ito, H., & Kanno, I. (2013). Hypoxia-induced cerebral angiogenesis in mouse cortex with two-photon microscopy. In Oxygen Transport to Tissue XXXV (pp. 15-20). (Advances in Experimental Medicine and Biology; Vol. 789). Springer New York LLC. https://doi.org/10.1007/978-1-4614-7411-1_3