Hypoxia-induced cerebral angiogenesis in mouse cortex with two-photon microscopy

Kazuto Masamoto, Hiroyuki Takuwa, Yutaka Tomita, Haruki Toriumi, Miyuki Unekawa, Junko Taniguchi, Hiroshi Kawaguchi, Yoshiaki Itoh, Norihiro Suzuki, Hiroshi Ito, Iwao Kanno

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

To better understand cellular interactions of the cerebral angiogenesis induced by hypoxia, a spatiotemporal dynamics of cortical microvascular restructuring during an exposure to continuous hypoxia was characterized with in vivo two-photon microscopy in mouse cortex. The mice were prepared with a closed cranial window over the sensory-motor cortex and housed in 8-9 % oxygen room for 2-4 weeks. Before beginning the hypoxic exposure, two-photon imaging of cortical microvasculature was performed, and the follow-up imaging was conducted weekly in the identical locations. We observed that 1-2 weeks after the onset of hypoxic exposure, a sprouting of new vessels appeared from the existing capillaries. An average emergence rate of the new vessel was 15 vessels per unit volume (mm<sup>3</sup>). The highest emergence rate was found in the cortical depths of 100-200 μm, indicating no spatial uniformity among the cortical layers. Further, a leakage of fluorescent dye (sulforhodamine 101) injected into the bloodstream was not detected, suggesting that the blood-brain barrier (BBB) was maintained. Future studies are needed to elucidate the roles of perivascular cells (e.g., pericyte, microglia, and astroglia) in a process of this hypoxia-induced angiogenesis, such as sprouting, growth, and merger with the existing capillary networks, while maintaining the BBB.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages15-20
Number of pages6
Volume789
ISBN (Print)9781461472568
DOIs
Publication statusPublished - 2013

Publication series

NameAdvances in Experimental Medicine and Biology
Volume789
ISSN (Print)00652598

Fingerprint

Brain Hypoxia
Photons
Microscopy
Microscopic examination
Blood-Brain Barrier
Imaging techniques
Fluorescent Dyes
Pericytes
Microglia
Oxygen
Microvessels
Astrocytes
Growth
Hypoxia
sulforhodamine 101

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Masamoto, K., Takuwa, H., Tomita, Y., Toriumi, H., Unekawa, M., Taniguchi, J., ... Kanno, I. (2013). Hypoxia-induced cerebral angiogenesis in mouse cortex with two-photon microscopy. In Advances in Experimental Medicine and Biology (Vol. 789, pp. 15-20). (Advances in Experimental Medicine and Biology; Vol. 789). Springer New York LLC. https://doi.org/10.1007/978-1-4614-7411-1_3

Hypoxia-induced cerebral angiogenesis in mouse cortex with two-photon microscopy. / Masamoto, Kazuto; Takuwa, Hiroyuki; Tomita, Yutaka; Toriumi, Haruki; Unekawa, Miyuki; Taniguchi, Junko; Kawaguchi, Hiroshi; Itoh, Yoshiaki; Suzuki, Norihiro; Ito, Hiroshi; Kanno, Iwao.

Advances in Experimental Medicine and Biology. Vol. 789 Springer New York LLC, 2013. p. 15-20 (Advances in Experimental Medicine and Biology; Vol. 789).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Masamoto, K, Takuwa, H, Tomita, Y, Toriumi, H, Unekawa, M, Taniguchi, J, Kawaguchi, H, Itoh, Y, Suzuki, N, Ito, H & Kanno, I 2013, Hypoxia-induced cerebral angiogenesis in mouse cortex with two-photon microscopy. in Advances in Experimental Medicine and Biology. vol. 789, Advances in Experimental Medicine and Biology, vol. 789, Springer New York LLC, pp. 15-20. https://doi.org/10.1007/978-1-4614-7411-1_3
Masamoto K, Takuwa H, Tomita Y, Toriumi H, Unekawa M, Taniguchi J et al. Hypoxia-induced cerebral angiogenesis in mouse cortex with two-photon microscopy. In Advances in Experimental Medicine and Biology. Vol. 789. Springer New York LLC. 2013. p. 15-20. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-1-4614-7411-1_3
Masamoto, Kazuto ; Takuwa, Hiroyuki ; Tomita, Yutaka ; Toriumi, Haruki ; Unekawa, Miyuki ; Taniguchi, Junko ; Kawaguchi, Hiroshi ; Itoh, Yoshiaki ; Suzuki, Norihiro ; Ito, Hiroshi ; Kanno, Iwao. / Hypoxia-induced cerebral angiogenesis in mouse cortex with two-photon microscopy. Advances in Experimental Medicine and Biology. Vol. 789 Springer New York LLC, 2013. pp. 15-20 (Advances in Experimental Medicine and Biology).
@inproceedings{1e58ab90709e4edd9fd6569cad087d13,
title = "Hypoxia-induced cerebral angiogenesis in mouse cortex with two-photon microscopy",
abstract = "To better understand cellular interactions of the cerebral angiogenesis induced by hypoxia, a spatiotemporal dynamics of cortical microvascular restructuring during an exposure to continuous hypoxia was characterized with in vivo two-photon microscopy in mouse cortex. The mice were prepared with a closed cranial window over the sensory-motor cortex and housed in 8-9 {\%} oxygen room for 2-4 weeks. Before beginning the hypoxic exposure, two-photon imaging of cortical microvasculature was performed, and the follow-up imaging was conducted weekly in the identical locations. We observed that 1-2 weeks after the onset of hypoxic exposure, a sprouting of new vessels appeared from the existing capillaries. An average emergence rate of the new vessel was 15 vessels per unit volume (mm3). The highest emergence rate was found in the cortical depths of 100-200 μm, indicating no spatial uniformity among the cortical layers. Further, a leakage of fluorescent dye (sulforhodamine 101) injected into the bloodstream was not detected, suggesting that the blood-brain barrier (BBB) was maintained. Future studies are needed to elucidate the roles of perivascular cells (e.g., pericyte, microglia, and astroglia) in a process of this hypoxia-induced angiogenesis, such as sprouting, growth, and merger with the existing capillary networks, while maintaining the BBB.",
author = "Kazuto Masamoto and Hiroyuki Takuwa and Yutaka Tomita and Haruki Toriumi and Miyuki Unekawa and Junko Taniguchi and Hiroshi Kawaguchi and Yoshiaki Itoh and Norihiro Suzuki and Hiroshi Ito and Iwao Kanno",
year = "2013",
doi = "10.1007/978-1-4614-7411-1_3",
language = "English",
isbn = "9781461472568",
volume = "789",
series = "Advances in Experimental Medicine and Biology",
publisher = "Springer New York LLC",
pages = "15--20",
booktitle = "Advances in Experimental Medicine and Biology",

}

TY - GEN

T1 - Hypoxia-induced cerebral angiogenesis in mouse cortex with two-photon microscopy

AU - Masamoto, Kazuto

AU - Takuwa, Hiroyuki

AU - Tomita, Yutaka

AU - Toriumi, Haruki

AU - Unekawa, Miyuki

AU - Taniguchi, Junko

AU - Kawaguchi, Hiroshi

AU - Itoh, Yoshiaki

AU - Suzuki, Norihiro

AU - Ito, Hiroshi

AU - Kanno, Iwao

PY - 2013

Y1 - 2013

N2 - To better understand cellular interactions of the cerebral angiogenesis induced by hypoxia, a spatiotemporal dynamics of cortical microvascular restructuring during an exposure to continuous hypoxia was characterized with in vivo two-photon microscopy in mouse cortex. The mice were prepared with a closed cranial window over the sensory-motor cortex and housed in 8-9 % oxygen room for 2-4 weeks. Before beginning the hypoxic exposure, two-photon imaging of cortical microvasculature was performed, and the follow-up imaging was conducted weekly in the identical locations. We observed that 1-2 weeks after the onset of hypoxic exposure, a sprouting of new vessels appeared from the existing capillaries. An average emergence rate of the new vessel was 15 vessels per unit volume (mm3). The highest emergence rate was found in the cortical depths of 100-200 μm, indicating no spatial uniformity among the cortical layers. Further, a leakage of fluorescent dye (sulforhodamine 101) injected into the bloodstream was not detected, suggesting that the blood-brain barrier (BBB) was maintained. Future studies are needed to elucidate the roles of perivascular cells (e.g., pericyte, microglia, and astroglia) in a process of this hypoxia-induced angiogenesis, such as sprouting, growth, and merger with the existing capillary networks, while maintaining the BBB.

AB - To better understand cellular interactions of the cerebral angiogenesis induced by hypoxia, a spatiotemporal dynamics of cortical microvascular restructuring during an exposure to continuous hypoxia was characterized with in vivo two-photon microscopy in mouse cortex. The mice were prepared with a closed cranial window over the sensory-motor cortex and housed in 8-9 % oxygen room for 2-4 weeks. Before beginning the hypoxic exposure, two-photon imaging of cortical microvasculature was performed, and the follow-up imaging was conducted weekly in the identical locations. We observed that 1-2 weeks after the onset of hypoxic exposure, a sprouting of new vessels appeared from the existing capillaries. An average emergence rate of the new vessel was 15 vessels per unit volume (mm3). The highest emergence rate was found in the cortical depths of 100-200 μm, indicating no spatial uniformity among the cortical layers. Further, a leakage of fluorescent dye (sulforhodamine 101) injected into the bloodstream was not detected, suggesting that the blood-brain barrier (BBB) was maintained. Future studies are needed to elucidate the roles of perivascular cells (e.g., pericyte, microglia, and astroglia) in a process of this hypoxia-induced angiogenesis, such as sprouting, growth, and merger with the existing capillary networks, while maintaining the BBB.

UR - http://www.scopus.com/inward/record.url?scp=84934444875&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84934444875&partnerID=8YFLogxK

U2 - 10.1007/978-1-4614-7411-1_3

DO - 10.1007/978-1-4614-7411-1_3

M3 - Conference contribution

SN - 9781461472568

VL - 789

T3 - Advances in Experimental Medicine and Biology

SP - 15

EP - 20

BT - Advances in Experimental Medicine and Biology

PB - Springer New York LLC

ER -