Hypoxia-inducible factor regulates expression of surfactant protein in alveolar type II cells in vitro

Yoko Ito, Aftab Ahmad, Emily Kewley, Robert J. Mason

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Alveolar type II (ATII) cells cultured at an air-liquid (A/L) interface maintain differentiation, but they lose these properties when they are submerged. Others showed that an oxygen tension gradient develops in the culture medium as ATII cells consume oxygen. Therefore, we wondered whether hypoxia inducible factor (HIF) signaling could explain differences in the phenotypes of ATII cells cultured under A/L interface or submerged conditions. ATII cells were isolated from male Sprague-Dawley rats and cultured on inserts coated with a mixture of rat-tail collagen and Matrigel, in medium including 5% rat serum and 10 ng/ml keratinocyte growth factor, with their apical surfaces either exposed to air or submerged. The A/L interface condition maintained the expression of surfactant proteins, whereas that expression was down-regulated under the submergedcondition, and the effect was rapid and reversible. Under submerged conditions, there was an increase in HIF1α and HIF2α in nuclear extracts, mRNA levels of HIF inducible genes, vascular endothelial growth factor, glucose transporter - 1 (GLUT1), and the protein level of pyruvate dehydrogenase kinase isozyme - 1. The expression of surfactant proteins was suppressed and GLUT1 mRNA levels were induced when cells were cultured with 1 mM dimethyloxalyl glycine. The expression of surfactant proteins was restored under submerged conditions with supplemented 60% oxygen. HIF signaling and oxygen tension at the surface of cells appears to be important in regulating the phenotype of rat ATII cells.

Original languageEnglish
Pages (from-to)938-945
Number of pages8
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume45
Issue number5
DOIs
Publication statusPublished - 2011 Nov 1

Fingerprint

Alveolar Epithelial Cells
Surface-Active Agents
Rats
Oxygen
Air
Facilitative Glucose Transport Proteins
Liquids
Proteins
Fibroblast Growth Factor 7
Messenger RNA
Die casting inserts
Phenotype
Surface Tension
Glycine
Vascular Endothelial Growth Factor A
Culture Media
Collagen
Genes
Sprague Dawley Rats
Tail

Keywords

  • AtII cells
  • GLUT1
  • HIF
  • Surfactant proteins
  • VEGF

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Hypoxia-inducible factor regulates expression of surfactant protein in alveolar type II cells in vitro. / Ito, Yoko; Ahmad, Aftab; Kewley, Emily; Mason, Robert J.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 45, No. 5, 01.11.2011, p. 938-945.

Research output: Contribution to journalArticle

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