Hzf regulates adipogenesis through translational control of C/EBPα

Hiroyuki Kawagishi, Takeshi Wakoh, Hatsume Uno, Mitsuo Maruyama, Ayako Moriya, Satoru Morikawa, Hideyuki Okano, Charles J. Sherr, Masatoshi Takagi, Masataka Sugimoto

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Adipocyte differentiation requires a well-defined programme of gene expression in which the transcription factor C/EBPα (CCAAT/enhancer- binding protein) has a central function. Here, we show that Hzf (haematopoietic zinc-finger), a previously identified p53 transcriptional target, regulates C/EBPα expression. Hzf is induced during differentiation of preadipocyte cell lines, and its suppression by short hairpin RNA disrupts adipogenesis. In Hzf's absence, expression of C/EBPα is severely impaired because of reduced translation of its mRNA. Hzf physically interacts with the 3′ untranslated region of C/EBPα mRNA to enhance its translation. Taken together, these findings underscore a critical role of Hzf in the adipogenesis regulatory cascade.

Original languageEnglish
Pages (from-to)1481-1490
Number of pages10
JournalEMBO Journal
Volume27
Issue number10
DOIs
Publication statusPublished - 2008 May 21

Fingerprint

CCAAT-Enhancer-Binding Proteins
Adipogenesis
Zinc Fingers
Zinc
Messenger RNA
Protein Biosynthesis
3' Untranslated Regions
Adipocytes
Gene expression
Small Interfering RNA
Transcription Factors
Cells
Gene Expression
Cell Line

Keywords

  • Adipocyte
  • C/EBPα
  • Hzf
  • Translation

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Kawagishi, H., Wakoh, T., Uno, H., Maruyama, M., Moriya, A., Morikawa, S., ... Sugimoto, M. (2008). Hzf regulates adipogenesis through translational control of C/EBPα. EMBO Journal, 27(10), 1481-1490. https://doi.org/10.1038/emboj.2008.76

Hzf regulates adipogenesis through translational control of C/EBPα. / Kawagishi, Hiroyuki; Wakoh, Takeshi; Uno, Hatsume; Maruyama, Mitsuo; Moriya, Ayako; Morikawa, Satoru; Okano, Hideyuki; Sherr, Charles J.; Takagi, Masatoshi; Sugimoto, Masataka.

In: EMBO Journal, Vol. 27, No. 10, 21.05.2008, p. 1481-1490.

Research output: Contribution to journalArticle

Kawagishi, H, Wakoh, T, Uno, H, Maruyama, M, Moriya, A, Morikawa, S, Okano, H, Sherr, CJ, Takagi, M & Sugimoto, M 2008, 'Hzf regulates adipogenesis through translational control of C/EBPα', EMBO Journal, vol. 27, no. 10, pp. 1481-1490. https://doi.org/10.1038/emboj.2008.76
Kawagishi H, Wakoh T, Uno H, Maruyama M, Moriya A, Morikawa S et al. Hzf regulates adipogenesis through translational control of C/EBPα. EMBO Journal. 2008 May 21;27(10):1481-1490. https://doi.org/10.1038/emboj.2008.76
Kawagishi, Hiroyuki ; Wakoh, Takeshi ; Uno, Hatsume ; Maruyama, Mitsuo ; Moriya, Ayako ; Morikawa, Satoru ; Okano, Hideyuki ; Sherr, Charles J. ; Takagi, Masatoshi ; Sugimoto, Masataka. / Hzf regulates adipogenesis through translational control of C/EBPα. In: EMBO Journal. 2008 ; Vol. 27, No. 10. pp. 1481-1490.
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