Hzf regulates adipogenesis through translational control of C/EBPα

Hiroyuki Kawagishi, Takeshi Wakoh, Hatsume Uno, Mitsuo Maruyama, Ayako Moriya, Satoru Morikawa, Hideyuki Okano, Charles J. Sherr, Masatoshi Takagi, Masataka Sugimoto

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Adipocyte differentiation requires a well-defined programme of gene expression in which the transcription factor C/EBPα (CCAAT/enhancer- binding protein) has a central function. Here, we show that Hzf (haematopoietic zinc-finger), a previously identified p53 transcriptional target, regulates C/EBPα expression. Hzf is induced during differentiation of preadipocyte cell lines, and its suppression by short hairpin RNA disrupts adipogenesis. In Hzf's absence, expression of C/EBPα is severely impaired because of reduced translation of its mRNA. Hzf physically interacts with the 3′ untranslated region of C/EBPα mRNA to enhance its translation. Taken together, these findings underscore a critical role of Hzf in the adipogenesis regulatory cascade.

Original languageEnglish
Pages (from-to)1481-1490
Number of pages10
JournalEMBO Journal
Volume27
Issue number10
DOIs
Publication statusPublished - 2008 May 21

Keywords

  • Adipocyte
  • C/EBPα
  • Hzf
  • Translation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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  • Cite this

    Kawagishi, H., Wakoh, T., Uno, H., Maruyama, M., Moriya, A., Morikawa, S., Okano, H., Sherr, C. J., Takagi, M., & Sugimoto, M. (2008). Hzf regulates adipogenesis through translational control of C/EBPα. EMBO Journal, 27(10), 1481-1490. https://doi.org/10.1038/emboj.2008.76