Identification and characterization of a new pair of immunoglobulin-like receptors LMIR1 and 2 derived from murine bone marrow-derived mast cells

Hidetoshi Kumagai, Toshihiko Oki, Kaori Tamitsu, Si Zhou Feng, Masao Ono, Hideaki Nakajima, Ying Chun Bao, Yuko Kawakami, Kazunari Nagayoshi, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Toshiaki Kawakami, Toshio Kitamura

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

We have identified and characterized two mouse cDNAs in a mouse antigen-stimulated bone marrow-derived mast cell cDNA library, both of which encode type I transmembrane proteins. The genes were closely mapped in the distal region of mouse chromosome 11 and expressed not only in mast cells but also widely in leukocytes. The extracellular domains of their encoded proteins contain a single variable immunoglobulin (Ig) motif sharing about 90% identity with amino acids, showing that they comprise a pair of molecules and belong to the Ig superfamily. We named these molecules leukocyte mono-Ig-like receptor1 and 2 (LMIR1 and 2). The intracellular domain of LMIR1 contains several immunoreceptor tyrosine-based inhibition motifs (ITIMs). When cross-linked, the intracellular domain was tyrosine phosphorylated and capable of recruiting tyrosine phosphatases, SHP-1 and SHP-2 and inositol polyphosphate 5-phosphatase, SHIP. LMIR2, on the other hand, contains a short cytoplasmic tail and a characteristic transmembrane domain carrying two positively charged amino acids associated with three kinds of immunoreceptor tyrosine-based activation motif (ITAM)-bearing molecules, DAP10, DAP12, and FcRγ. These findings suggest that a new pair of ITIM/ITAM-bearing receptors, LMIR1 and 2, regulate mast cell-mediated inflammatory responses through yet to be defined ligand(s).

Original languageEnglish
Pages (from-to)719-729
Number of pages11
JournalBiochemical and Biophysical Research Communications
Volume307
Issue number3
DOIs
Publication statusPublished - 2003 Aug 1
Externally publishedYes

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Immunoreceptor Tyrosine-Based Inhibition Motif
Immunoreceptor Tyrosine-Based Activation Motif
Mast Cells
Tyrosine
Immunoglobulins
Bone
Leukocytes
Bone Marrow
Bearings (structural)
Non-Receptor Type 6 Protein Tyrosine Phosphatase
Amino Acids
Chromosomes, Human, Pair 11
Molecules
Gene Library
Chemical activation
Tail
Proteins
Complementary DNA
Polyphosphates
Ligands

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification and characterization of a new pair of immunoglobulin-like receptors LMIR1 and 2 derived from murine bone marrow-derived mast cells. / Kumagai, Hidetoshi; Oki, Toshihiko; Tamitsu, Kaori; Feng, Si Zhou; Ono, Masao; Nakajima, Hideaki; Bao, Ying Chun; Kawakami, Yuko; Nagayoshi, Kazunari; Copeland, Neal G.; Gilbert, Debra J.; Jenkins, Nancy A.; Kawakami, Toshiaki; Kitamura, Toshio.

In: Biochemical and Biophysical Research Communications, Vol. 307, No. 3, 01.08.2003, p. 719-729.

Research output: Contribution to journalArticle

Kumagai, H, Oki, T, Tamitsu, K, Feng, SZ, Ono, M, Nakajima, H, Bao, YC, Kawakami, Y, Nagayoshi, K, Copeland, NG, Gilbert, DJ, Jenkins, NA, Kawakami, T & Kitamura, T 2003, 'Identification and characterization of a new pair of immunoglobulin-like receptors LMIR1 and 2 derived from murine bone marrow-derived mast cells', Biochemical and Biophysical Research Communications, vol. 307, no. 3, pp. 719-729. https://doi.org/10.1016/S0006-291X(03)01245-2
Kumagai, Hidetoshi ; Oki, Toshihiko ; Tamitsu, Kaori ; Feng, Si Zhou ; Ono, Masao ; Nakajima, Hideaki ; Bao, Ying Chun ; Kawakami, Yuko ; Nagayoshi, Kazunari ; Copeland, Neal G. ; Gilbert, Debra J. ; Jenkins, Nancy A. ; Kawakami, Toshiaki ; Kitamura, Toshio. / Identification and characterization of a new pair of immunoglobulin-like receptors LMIR1 and 2 derived from murine bone marrow-derived mast cells. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 307, No. 3. pp. 719-729.
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AU - Feng, Si Zhou

AU - Ono, Masao

AU - Nakajima, Hideaki

AU - Bao, Ying Chun

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AU - Nagayoshi, Kazunari

AU - Copeland, Neal G.

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