Identification and characterization of stem cells in prepubertal spermatogenesis in mice

Kazuyuki Ohbo, Shosei Yoshida, Masako Ohmura, Osamu Ohneda, Takehiko Ogawa, Hideaki Tsuchiya, Takashi Kuwana, James Kehler, Kuniya Abe, Hans R. Schöler, Toshio Suda

Research output: Contribution to journalArticle

174 Citations (Scopus)

Abstract

The stem cell properties of gonocytes and prospermatogonia at prepubertal stages are still largely unknown: it is not clear whether gonocytes and prospermatogonia are a special cell type or similar to adult undifferentiated spermatogonia. To characterize these cells, we have established transgenic mice carrying EGFP (enhanced green fluorescence protein) cDNA under control of an Oct4 18-kb genomic fragment containing the minimal promoter and proximal and distal enhancers; Oct4 is reported to be expressed in undifferentiated spermatogonia at prepubertal stages. Generation of transgenic mice enabled us to purify gonocytes and prospermatogonia from the somatic cells of the testis. Transplantation studies of testicular cells so far have been done with a mixture of germ cells and somatic cells. This is the first report that establishes how to purify germ cells from total testicular cells, enabling evaluation of cell-autonomous repopulating activity of a subpopulation of prospermatogonia. We show that prospermatogonia differ markedly from adult spermatogonia in both the size of the KIT-negative population and cell cycle characteristics. The GFP+KIT- fraction of prospermatogonia has much higher repopulating activity than does the GFP+KIT+ population in the adult environment. Interestingly, the GFP+KIT+ population still exhibits repopulating activity, unlike adult KIT-positive spermatogonia. We also show that ALCAM, activated leukocyte cell adhesion molecule, is expressed transiently in gonocytes. Sertoli cells and myoid cells also express ALCAM at the same stage, suggesting that ALCAM may contribute to gonocyte-Sertoli cell adhesion and migration of gonoyctes toward the basement membrane.

Original languageEnglish
Pages (from-to)209-225
Number of pages17
JournalDevelopmental Biology
Volume258
Issue number1
DOIs
Publication statusPublished - 2003 Jun 1

Keywords

  • Activated leukocyte cell adhesion molecule
  • GFP
  • KIT
  • Oct4
  • Prespermatogenesis
  • Prospermatogonia
  • Transgenic mice

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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    Ohbo, K., Yoshida, S., Ohmura, M., Ohneda, O., Ogawa, T., Tsuchiya, H., Kuwana, T., Kehler, J., Abe, K., Schöler, H. R., & Suda, T. (2003). Identification and characterization of stem cells in prepubertal spermatogenesis in mice. Developmental Biology, 258(1), 209-225. https://doi.org/10.1016/S0012-1606(03)00111-8