Identification and characterization of the high-affinity choline transporter

Takashi Okuda, Tatsuya Haga, Yoshikatsu Kanai, Hitoshi Endou, Takeshi Ishihara, Isao Katsura

Research output: Contribution to journalArticle

241 Citations (Scopus)

Abstract

In cholinergic neurons, high-affinity choline uptake in presynaptic terminals is the rate-limiting step in acetylcholine synthesis. Using information provided by the Caenorhabditis elegans Genome Project, we cloned a cDNA encoding the high-affinity choline transporter from C. elegans (cho- 1). We subsequently used this clone to isolate the corresponding cDNA from rat (CHT1). CHT1 is not homologous to neurotransmitter transporters, but is homologous to members of the Na+-dependent glucose transporter family. Expression of CHT1 mRNA is restricted to cholinergic neurons. The characteristics of CHT1-mediated choline uptake essentially match those of high-affinity choline uptake in rat brain synaptosomes.

Original languageEnglish
Pages (from-to)120-125
Number of pages6
JournalNature Neuroscience
Volume3
Issue number2
DOIs
Publication statusPublished - 2000 Feb
Externally publishedYes

Fingerprint

Choline
Cholinergic Neurons
Caenorhabditis elegans
Complementary DNA
Neurotransmitter Transport Proteins
Facilitative Glucose Transport Proteins
Synaptosomes
Presynaptic Terminals
Acetylcholine
Clone Cells
Genome
Messenger RNA
Brain
choline transporter

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Okuda, T., Haga, T., Kanai, Y., Endou, H., Ishihara, T., & Katsura, I. (2000). Identification and characterization of the high-affinity choline transporter. Nature Neuroscience, 3(2), 120-125. https://doi.org/10.1038/72059

Identification and characterization of the high-affinity choline transporter. / Okuda, Takashi; Haga, Tatsuya; Kanai, Yoshikatsu; Endou, Hitoshi; Ishihara, Takeshi; Katsura, Isao.

In: Nature Neuroscience, Vol. 3, No. 2, 02.2000, p. 120-125.

Research output: Contribution to journalArticle

Okuda, T, Haga, T, Kanai, Y, Endou, H, Ishihara, T & Katsura, I 2000, 'Identification and characterization of the high-affinity choline transporter', Nature Neuroscience, vol. 3, no. 2, pp. 120-125. https://doi.org/10.1038/72059
Okuda, Takashi ; Haga, Tatsuya ; Kanai, Yoshikatsu ; Endou, Hitoshi ; Ishihara, Takeshi ; Katsura, Isao. / Identification and characterization of the high-affinity choline transporter. In: Nature Neuroscience. 2000 ; Vol. 3, No. 2. pp. 120-125.
@article{bd32fe863e32494795196849faf280c0,
title = "Identification and characterization of the high-affinity choline transporter",
abstract = "In cholinergic neurons, high-affinity choline uptake in presynaptic terminals is the rate-limiting step in acetylcholine synthesis. Using information provided by the Caenorhabditis elegans Genome Project, we cloned a cDNA encoding the high-affinity choline transporter from C. elegans (cho- 1). We subsequently used this clone to isolate the corresponding cDNA from rat (CHT1). CHT1 is not homologous to neurotransmitter transporters, but is homologous to members of the Na+-dependent glucose transporter family. Expression of CHT1 mRNA is restricted to cholinergic neurons. The characteristics of CHT1-mediated choline uptake essentially match those of high-affinity choline uptake in rat brain synaptosomes.",
author = "Takashi Okuda and Tatsuya Haga and Yoshikatsu Kanai and Hitoshi Endou and Takeshi Ishihara and Isao Katsura",
year = "2000",
month = "2",
doi = "10.1038/72059",
language = "English",
volume = "3",
pages = "120--125",
journal = "Nature Neuroscience",
issn = "1097-6256",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Identification and characterization of the high-affinity choline transporter

AU - Okuda, Takashi

AU - Haga, Tatsuya

AU - Kanai, Yoshikatsu

AU - Endou, Hitoshi

AU - Ishihara, Takeshi

AU - Katsura, Isao

PY - 2000/2

Y1 - 2000/2

N2 - In cholinergic neurons, high-affinity choline uptake in presynaptic terminals is the rate-limiting step in acetylcholine synthesis. Using information provided by the Caenorhabditis elegans Genome Project, we cloned a cDNA encoding the high-affinity choline transporter from C. elegans (cho- 1). We subsequently used this clone to isolate the corresponding cDNA from rat (CHT1). CHT1 is not homologous to neurotransmitter transporters, but is homologous to members of the Na+-dependent glucose transporter family. Expression of CHT1 mRNA is restricted to cholinergic neurons. The characteristics of CHT1-mediated choline uptake essentially match those of high-affinity choline uptake in rat brain synaptosomes.

AB - In cholinergic neurons, high-affinity choline uptake in presynaptic terminals is the rate-limiting step in acetylcholine synthesis. Using information provided by the Caenorhabditis elegans Genome Project, we cloned a cDNA encoding the high-affinity choline transporter from C. elegans (cho- 1). We subsequently used this clone to isolate the corresponding cDNA from rat (CHT1). CHT1 is not homologous to neurotransmitter transporters, but is homologous to members of the Na+-dependent glucose transporter family. Expression of CHT1 mRNA is restricted to cholinergic neurons. The characteristics of CHT1-mediated choline uptake essentially match those of high-affinity choline uptake in rat brain synaptosomes.

UR - http://www.scopus.com/inward/record.url?scp=0034004554&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034004554&partnerID=8YFLogxK

U2 - 10.1038/72059

DO - 10.1038/72059

M3 - Article

VL - 3

SP - 120

EP - 125

JO - Nature Neuroscience

JF - Nature Neuroscience

SN - 1097-6256

IS - 2

ER -