Identification by differential tissue proteome analysis of Talin-1 as a novel molecular marker of progression of hepatocellular carcinoma

Hideaki Kanamori, Takao Kawakami, Kathryn Effendi, Ken Yamazaki, Taisuke Mori, Hirotoshi Ebinuma, Yohei Masugi, Wenlin Du, Keiko Nagasaka, Atsushi Ogiwara, Yutaka Kyono, Minoru Tanabe, Hidetsugu Saito, Toshifumi Hibi, Michiie Sakamoto

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Objective: Hepatocellular carcinoma (HCC) is characterized by a multistage process of tumor progression. This study addressed its molecular features to identify novel protein candidates involved in HCC progression. Methods: Using liquid chromatography-tandem mass spectrometry, proteomes of 4 early HCCs and 4 non-HCC tissues derived from 2 cases of liver transplant surgery were compared with respect to the separation profiles of their tryptic peptides. Immunohistochemistry was performed on 106 HCC nodules to confirm the results of the proteomic analysis. Results: Statistical analysis of the profiles selected the peptide peaks differentiating HCC from non-HCC. A database search of the tandem mass spectrometry data from those peptide peaks identified 61 proteins, including a cytoskeletal protein, talin-1, as upregulated in HCC. Talin-1 expression levels in HCC nodules were significantly associated with the dedifferentiation of HCC (p = 0.001). A follow-up survey of the examined clinical cases revealed a correlation between talin-1 upregulation and a shorter time to recurrence after resection (p = 0.039), which may be related to the higher rate of portal vein invasion in HCCs with talin-1 up-regulation (p = 0.029). Conclusions: Proteomic analysis led to identification of talin-1 as a promising HCC marker. Talin-1 upregulation is associated with HCC progression and may serve as a prognostic marker.

Original languageEnglish
Pages (from-to)406-415
Number of pages10
JournalOncology
Volume80
Issue number5-6
DOIs
Publication statusPublished - 2011 Aug

Fingerprint

Talin
Proteome
Hepatocellular Carcinoma
Up-Regulation
Tandem Mass Spectrometry
Proteomics
Peptides
Carcinoma
Cytoskeletal Proteins
Portal Vein
Liquid Chromatography
Proteins
Immunohistochemistry
Databases
Transplants

Keywords

  • Disease-free survival
  • Immunohistochemistry
  • Liquid chromatography-tandem mass spectrometry
  • Portal vein invasion
  • Tumor progression

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Identification by differential tissue proteome analysis of Talin-1 as a novel molecular marker of progression of hepatocellular carcinoma. / Kanamori, Hideaki; Kawakami, Takao; Effendi, Kathryn; Yamazaki, Ken; Mori, Taisuke; Ebinuma, Hirotoshi; Masugi, Yohei; Du, Wenlin; Nagasaka, Keiko; Ogiwara, Atsushi; Kyono, Yutaka; Tanabe, Minoru; Saito, Hidetsugu; Hibi, Toshifumi; Sakamoto, Michiie.

In: Oncology, Vol. 80, No. 5-6, 08.2011, p. 406-415.

Research output: Contribution to journalArticle

Kanamori, H, Kawakami, T, Effendi, K, Yamazaki, K, Mori, T, Ebinuma, H, Masugi, Y, Du, W, Nagasaka, K, Ogiwara, A, Kyono, Y, Tanabe, M, Saito, H, Hibi, T & Sakamoto, M 2011, 'Identification by differential tissue proteome analysis of Talin-1 as a novel molecular marker of progression of hepatocellular carcinoma', Oncology, vol. 80, no. 5-6, pp. 406-415. https://doi.org/10.1159/000330734
Kanamori, Hideaki ; Kawakami, Takao ; Effendi, Kathryn ; Yamazaki, Ken ; Mori, Taisuke ; Ebinuma, Hirotoshi ; Masugi, Yohei ; Du, Wenlin ; Nagasaka, Keiko ; Ogiwara, Atsushi ; Kyono, Yutaka ; Tanabe, Minoru ; Saito, Hidetsugu ; Hibi, Toshifumi ; Sakamoto, Michiie. / Identification by differential tissue proteome analysis of Talin-1 as a novel molecular marker of progression of hepatocellular carcinoma. In: Oncology. 2011 ; Vol. 80, No. 5-6. pp. 406-415.
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AU - Kawakami, Takao

AU - Effendi, Kathryn

AU - Yamazaki, Ken

AU - Mori, Taisuke

AU - Ebinuma, Hirotoshi

AU - Masugi, Yohei

AU - Du, Wenlin

AU - Nagasaka, Keiko

AU - Ogiwara, Atsushi

AU - Kyono, Yutaka

AU - Tanabe, Minoru

AU - Saito, Hidetsugu

AU - Hibi, Toshifumi

AU - Sakamoto, Michiie

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AB - Objective: Hepatocellular carcinoma (HCC) is characterized by a multistage process of tumor progression. This study addressed its molecular features to identify novel protein candidates involved in HCC progression. Methods: Using liquid chromatography-tandem mass spectrometry, proteomes of 4 early HCCs and 4 non-HCC tissues derived from 2 cases of liver transplant surgery were compared with respect to the separation profiles of their tryptic peptides. Immunohistochemistry was performed on 106 HCC nodules to confirm the results of the proteomic analysis. Results: Statistical analysis of the profiles selected the peptide peaks differentiating HCC from non-HCC. A database search of the tandem mass spectrometry data from those peptide peaks identified 61 proteins, including a cytoskeletal protein, talin-1, as upregulated in HCC. Talin-1 expression levels in HCC nodules were significantly associated with the dedifferentiation of HCC (p = 0.001). A follow-up survey of the examined clinical cases revealed a correlation between talin-1 upregulation and a shorter time to recurrence after resection (p = 0.039), which may be related to the higher rate of portal vein invasion in HCCs with talin-1 up-regulation (p = 0.029). Conclusions: Proteomic analysis led to identification of talin-1 as a promising HCC marker. Talin-1 upregulation is associated with HCC progression and may serve as a prognostic marker.

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