Identification, cDNA cloning, and targeted deletion of p70, a novel, ubiquitously expressed SH3 domain-containing protein

Nick Carpino, Ryuji Kobayashi, Heesuk Zang, Yutaka Takahashi, Shiann Tarrng Jou, Jian Feng, Hideaki Nakajima, James N. Ihle

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Abstract

In a screen for proteins that interact with Jak2, we identified a previously uncharacterized 70-kDa protein and cloned the corresponding cDNA. The predicated sequence indicates that p70 contains an SH3 domain and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. p70 transcripts were found in all tissues examined. Similarly, when an antibody raised against a C-terminal peptide to analyze p70 protein expression was used, all murine tissues examined were found to express p70. To investigate the in vivo role of p70, we generated a p70-deficient mouse strain. Mice lacking p70 are viable, develop normally, and do not display any obvious abnormalities. No differences were detected in various hematological parameters, including bone marrow colony-forming ability, in response to cytokines that utilize Jak2. In addition, no impairment in B- and T-cell development and proliferative ability was detected.

Original languageEnglish
Pages (from-to)7491-7500
Number of pages10
JournalMolecular and Cellular Biology
Volume22
Issue number21
DOIs
Publication statusPublished - 2002 Nov 1

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Carpino, N., Kobayashi, R., Zang, H., Takahashi, Y., Jou, S. T., Feng, J., Nakajima, H., & Ihle, J. N. (2002). Identification, cDNA cloning, and targeted deletion of p70, a novel, ubiquitously expressed SH3 domain-containing protein. Molecular and Cellular Biology, 22(21), 7491-7500. https://doi.org/10.1128/MCB.22.21.7491-7500.2002