Abstract
Sesamin is a furofuran-type lignan that is found abundantly in seeds of Sesamum indicum (sesame) and has been widely accepted as a dietary supplement with positive effects on human health. The biological activity of sesamin in human cells and organs has been analysed extensively, although comparatively few studies show biological functions for sesamin in planta. Herein we screened sesamin-binding proteins (SBP) from sesame seedling extracts using sesamin-immobilized nano-beads. In subsequent peptide mass fingerprinting analyses, we identified a SBP, Steroleosin B, which is one of the membrane proteins found in oil bodies. In addition, pull-down assays and saturation transfer difference-nuclear magnetic resonance (STD-NMR) experiments demonstrated that sesamin binds directly to recombinant Steroleosin B in vitro. Finally, ectopic accumulations of sesamin and Steroleosin B in transgenic Arabidopsis thaliana plants induced severe growth defects including suppression of leaf expansion and root elongation. Collectively, these results indicate that sesamin influences tissue development in the presence of Steroleosin B.
| Original language | English |
|---|---|
| Article number | 8631 |
| Journal | Scientific reports |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2019 Dec 1 |
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Identification of a binding protein for sesamin and characterization of its roles in plant growth. / Tera, Masayuki; Koyama, Tomotsugu; Murata, Jun; Furukawa, Ayako; Mori, Shoko; Azuma, Toshiaki; Watanabe, Takehiro; Hori, Katsuhito; Okazawa, Atsushi; Kabe, Yasuaki; Suematsu, Makoto; Satake, Honoo; Ono, Eiichiro; Horikawa, Manabu.
In: Scientific reports, Vol. 9, No. 1, 8631, 01.12.2019.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Identification of a binding protein for sesamin and characterization of its roles in plant growth
AU - Tera, Masayuki
AU - Koyama, Tomotsugu
AU - Murata, Jun
AU - Furukawa, Ayako
AU - Mori, Shoko
AU - Azuma, Toshiaki
AU - Watanabe, Takehiro
AU - Hori, Katsuhito
AU - Okazawa, Atsushi
AU - Kabe, Yasuaki
AU - Suematsu, Makoto
AU - Satake, Honoo
AU - Ono, Eiichiro
AU - Horikawa, Manabu
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Sesamin is a furofuran-type lignan that is found abundantly in seeds of Sesamum indicum (sesame) and has been widely accepted as a dietary supplement with positive effects on human health. The biological activity of sesamin in human cells and organs has been analysed extensively, although comparatively few studies show biological functions for sesamin in planta. Herein we screened sesamin-binding proteins (SBP) from sesame seedling extracts using sesamin-immobilized nano-beads. In subsequent peptide mass fingerprinting analyses, we identified a SBP, Steroleosin B, which is one of the membrane proteins found in oil bodies. In addition, pull-down assays and saturation transfer difference-nuclear magnetic resonance (STD-NMR) experiments demonstrated that sesamin binds directly to recombinant Steroleosin B in vitro. Finally, ectopic accumulations of sesamin and Steroleosin B in transgenic Arabidopsis thaliana plants induced severe growth defects including suppression of leaf expansion and root elongation. Collectively, these results indicate that sesamin influences tissue development in the presence of Steroleosin B.
AB - Sesamin is a furofuran-type lignan that is found abundantly in seeds of Sesamum indicum (sesame) and has been widely accepted as a dietary supplement with positive effects on human health. The biological activity of sesamin in human cells and organs has been analysed extensively, although comparatively few studies show biological functions for sesamin in planta. Herein we screened sesamin-binding proteins (SBP) from sesame seedling extracts using sesamin-immobilized nano-beads. In subsequent peptide mass fingerprinting analyses, we identified a SBP, Steroleosin B, which is one of the membrane proteins found in oil bodies. In addition, pull-down assays and saturation transfer difference-nuclear magnetic resonance (STD-NMR) experiments demonstrated that sesamin binds directly to recombinant Steroleosin B in vitro. Finally, ectopic accumulations of sesamin and Steroleosin B in transgenic Arabidopsis thaliana plants induced severe growth defects including suppression of leaf expansion and root elongation. Collectively, these results indicate that sesamin influences tissue development in the presence of Steroleosin B.
UR - http://www.scopus.com/inward/record.url?scp=85067291069&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85067291069&partnerID=8YFLogxK
U2 - 10.1038/s41598-019-45003-7
DO - 10.1038/s41598-019-45003-7
M3 - Article
C2 - 31201340
AN - SCOPUS:85067291069
VL - 9
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 8631
ER -