Identification of a binding protein for sesamin and characterization of its roles in plant growth

Masayuki Tera, Tomotsugu Koyama, Jun Murata, Ayako Furukawa, Shoko Mori, Toshiaki Azuma, Takehiro Watanabe, Katsuhito Hori, Atsushi Okazawa, Yasuaki Kabe, Makoto Suematsu, Honoo Satake, Eiichiro Ono, Manabu Horikawa

Research output: Contribution to journalArticle

Abstract

Sesamin is a furofuran-type lignan that is found abundantly in seeds of Sesamum indicum (sesame) and has been widely accepted as a dietary supplement with positive effects on human health. The biological activity of sesamin in human cells and organs has been analysed extensively, although comparatively few studies show biological functions for sesamin in planta. Herein we screened sesamin-binding proteins (SBP) from sesame seedling extracts using sesamin-immobilized nano-beads. In subsequent peptide mass fingerprinting analyses, we identified a SBP, Steroleosin B, which is one of the membrane proteins found in oil bodies. In addition, pull-down assays and saturation transfer difference-nuclear magnetic resonance (STD-NMR) experiments demonstrated that sesamin binds directly to recombinant Steroleosin B in vitro. Finally, ectopic accumulations of sesamin and Steroleosin B in transgenic Arabidopsis thaliana plants induced severe growth defects including suppression of leaf expansion and root elongation. Collectively, these results indicate that sesamin influences tissue development in the presence of Steroleosin B.

Original languageEnglish
Article number8631
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

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Carrier Proteins
Growth
Sesamum
sesamin
Lignans
Peptide Mapping
Dietary Supplements
Seedlings
Arabidopsis
Seeds
Membrane Proteins
Magnetic Resonance Spectroscopy
Health

ASJC Scopus subject areas

  • General

Cite this

Tera, M., Koyama, T., Murata, J., Furukawa, A., Mori, S., Azuma, T., ... Horikawa, M. (2019). Identification of a binding protein for sesamin and characterization of its roles in plant growth. Scientific reports, 9(1), [8631]. https://doi.org/10.1038/s41598-019-45003-7

Identification of a binding protein for sesamin and characterization of its roles in plant growth. / Tera, Masayuki; Koyama, Tomotsugu; Murata, Jun; Furukawa, Ayako; Mori, Shoko; Azuma, Toshiaki; Watanabe, Takehiro; Hori, Katsuhito; Okazawa, Atsushi; Kabe, Yasuaki; Suematsu, Makoto; Satake, Honoo; Ono, Eiichiro; Horikawa, Manabu.

In: Scientific reports, Vol. 9, No. 1, 8631, 01.12.2019.

Research output: Contribution to journalArticle

Tera, M, Koyama, T, Murata, J, Furukawa, A, Mori, S, Azuma, T, Watanabe, T, Hori, K, Okazawa, A, Kabe, Y, Suematsu, M, Satake, H, Ono, E & Horikawa, M 2019, 'Identification of a binding protein for sesamin and characterization of its roles in plant growth', Scientific reports, vol. 9, no. 1, 8631. https://doi.org/10.1038/s41598-019-45003-7
Tera, Masayuki ; Koyama, Tomotsugu ; Murata, Jun ; Furukawa, Ayako ; Mori, Shoko ; Azuma, Toshiaki ; Watanabe, Takehiro ; Hori, Katsuhito ; Okazawa, Atsushi ; Kabe, Yasuaki ; Suematsu, Makoto ; Satake, Honoo ; Ono, Eiichiro ; Horikawa, Manabu. / Identification of a binding protein for sesamin and characterization of its roles in plant growth. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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