Identification of a gene conferring resistance to zinc and cadmium ions in the yeast Saccharomyces cerevisiae

Akihito Kamizono, Masafumi Nishizawa, Yutaka Teranishi, Kousaku Murata, Akira Kimura

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

A DNA fragment conferring resistance to zinc and cadmium ions in the yeast Saccharomyces cerevisiae was isolated from a library of yeast genomic DNA. Its nucleotide sequence revealed the presence of a single open reading frame (ORF; 1326 bp) having the potential to encode a protein of 442 amino acid residues (molecular mass of 48.3 kDa). A frameshift mutation introduced within the ORF abolished resistance to heavy metal ions, indicating the ORF is required for resistance. Therefore, we termed it the ZRC1 (zinc resistance conferring) gene. The deduced amino acid sequence of the gene product predicts a rather hydrophobic protein with six possible membrane-spanning regions. While multiple copies of the ZRC1 gene enable yeast cells to grow in the presence of 40 mM Zn2+, a level at which wild-type cells cannot survive, the disruption of the chromosomal ZRC1 locus, though not a lethal event, makes cells more sensitive to zinc ions than are wild-type cells.

Original languageEnglish
Pages (from-to)161-167
Number of pages7
JournalMGG Molecular & General Genetics
Volume219
Issue number1-2
DOIs
Publication statusPublished - 1989 Oct

Fingerprint

Cadmium
Open Reading Frames
Saccharomyces cerevisiae
Zinc
Yeasts
Ions
Genes
Heavy Ions
Frameshift Mutation
Genomic Library
DNA
Heavy Metals
Amino Acid Sequence
Proteins
Amino Acids
Membranes

Keywords

  • DNA sequence
  • Heavy metal resistance
  • Membrane
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Genetics

Cite this

Identification of a gene conferring resistance to zinc and cadmium ions in the yeast Saccharomyces cerevisiae. / Kamizono, Akihito; Nishizawa, Masafumi; Teranishi, Yutaka; Murata, Kousaku; Kimura, Akira.

In: MGG Molecular & General Genetics, Vol. 219, No. 1-2, 10.1989, p. 161-167.

Research output: Contribution to journalArticle

Kamizono, Akihito ; Nishizawa, Masafumi ; Teranishi, Yutaka ; Murata, Kousaku ; Kimura, Akira. / Identification of a gene conferring resistance to zinc and cadmium ions in the yeast Saccharomyces cerevisiae. In: MGG Molecular & General Genetics. 1989 ; Vol. 219, No. 1-2. pp. 161-167.
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