Abstract
The induction of a mutant DnaA protein (DnaA E204Q) with decreased intrinsic ATPase activity in cells causes a lethal phenotype. Based on our results that the decreased ATPase activity of DnaA E204Q is activated to the level of a wild-type protein in the presence of partially purified stimulating factors for DnaA ATPase, we tested the hypothesis that genes encoding the stimulating factors can be cloned as high-copy-number plasmid suppressors for the lethality caused by DnaA E204Q. We isolated a number of high copy-number plasmids that suppress the lethal phenotype. The genes responsible for the suppression of the lethal phenotype were revealed to be dnaN, relA, pcnB and cyaA by subcloning, and a site-directed mutational analysis. The mechanisms by which these genes suppressed the lethal phenotype were examined in vivo and in vitro.
Original language | English |
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Pages (from-to) | 904-909 |
Number of pages | 6 |
Journal | Biological and Pharmaceutical Bulletin |
Volume | 22 |
Issue number | 9 |
DOIs | |
Publication status | Published - 1999 Sep |
Keywords
- ATPase
- DNA replication
- DnaA
- Suppressor
- The dnaN gene
ASJC Scopus subject areas
- Pharmacology
- Pharmaceutical Science