TY - JOUR
T1 - Identification of a molecular target of kurahyne, an apoptosis-inducing lipopeptide from marine cyanobacterial assemblages
AU - Iwasaki, Arihiro
AU - Ohno, Osamu
AU - Katsuyama, Shun
AU - Morita, Maho
AU - Sasazawa, Yukiko
AU - Dan, Shingo
AU - Simizu, Siro
AU - Yamori, Takao
AU - Suenaga, Kiyotake
N1 - Funding Information:
Special thanks to Dr. C. Toyoshima (University of Tokyo) for assessing SERCA-inhibitory activity. We thank Dr. Y. Kawazoe (Kanagawa University), Dr. K. Miyamoto (Keio University) and Mr. M. Kano (Keio University) for preparing the vector, pDsRed2-ER. This work was supported in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan ( 24310160 and 15K16555 ) for the Promotion of Science, Shorai Foundation for Science and Technology, and the Keio Gijuku Fukuzawa Memorial Fund for the Advancement of Education and Research.
Publisher Copyright:
© 2015 Elsevier Ltd. All rights reserved.
PY - 2015/11/15
Y1 - 2015/11/15
N2 - In 2014, we isolated kurahyne, an acetylene-containing lipopeptide, from a marine cyanobacterial assemblage of Lyngbya sp. Kurahyne exhibited growth-inhibitory activity against human cancer cells, and induced apoptosis in HeLa cells. However, its mode of action is not yet clear. To elucidate its mode of action, we carried out several cell-based assays, and identified the intracellular target molecule of kurahyne as sarco/endoplasmic reticulum Ca2+ ATPase (SERCA). In addition, we found that kurahyne inhibited the differentiation of macrophages into osteoclasts.
AB - In 2014, we isolated kurahyne, an acetylene-containing lipopeptide, from a marine cyanobacterial assemblage of Lyngbya sp. Kurahyne exhibited growth-inhibitory activity against human cancer cells, and induced apoptosis in HeLa cells. However, its mode of action is not yet clear. To elucidate its mode of action, we carried out several cell-based assays, and identified the intracellular target molecule of kurahyne as sarco/endoplasmic reticulum Ca2+ ATPase (SERCA). In addition, we found that kurahyne inhibited the differentiation of macrophages into osteoclasts.
KW - Apoptosis
KW - ER stress
KW - Kurahyne
KW - Marine natural product
KW - SERCA
UR - http://www.scopus.com/inward/record.url?scp=84945931562&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84945931562&partnerID=8YFLogxK
U2 - 10.1016/j.bmcl.2015.09.044
DO - 10.1016/j.bmcl.2015.09.044
M3 - Article
C2 - 26428873
AN - SCOPUS:84945931562
VL - 25
SP - 5295
EP - 5298
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
SN - 0960-894X
IS - 22
ER -
