Identification of a neuron-specific human gene, KIAA1110, that is a guanine nucleotide exchange factor for ARF1

Yujiro Hattori, Shigeki Ohta, Kenji Hamada, Hisafumi Yamada-Okabe, Yonehiro Kanemura, Yumi Matsuzaki, Hideyuki Okano, Yutaka Kawakami, Masahiro Toda

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

To identify neuron-specific genes, we performed gene expression profiling, cDNA microarray and in silico ESTs (expressed sequence tags) analyses. We identified a human neuron-specific gene, KIAA1110 (homologue of rat synArfGEF (Po)), that is a member of the guanine nucleotide exchange factor (GEF) for the ADP-ribosylation factor (ARF). RT-PCR analysis showed that the KIAA1110 gene was expressed specifically in the brain among adult human tissues, whereas no apparent expression was observed in immature neural tissues/cells, such as fetal brain, glioma tissues/cells, and neural stem/precursor cells (NSPCs). The KIAA1110 protein was shown to be expressed in mature neurons but not in undifferentiated NSPCs. Immunohistochemical analysis also showed that KIAA1110 was expressed in neurons of the human adult cerebral cortex. Furthermore, the pull-down assay revealed that KIAA1110 has a GEF activity toward ARF1 that regulates transport along the secretion pathway. These results suggest that KIAA1110 is expressed specifically in mature neurons and may play an important role in the secretion pathway as a GEF for ARF1.

Original languageEnglish
Pages (from-to)737-742
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume364
Issue number4
DOIs
Publication statusPublished - 2007 Dec 28

Keywords

  • ARF-GEFs
  • KIAA1110
  • Neural stem cells

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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