Identification of a novel aquaporin, AQP12, expressed in pancreatic acinar cells

Tomohiro Itoh, Tatemitsu Rai, Michio Kuwahara, Shigeru Ko, Shinichi Uchida, Sei Sasaki, Kenichi Ishibashi

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Members of the aquaporin (AQP) water channel family are widely distributed in various tissues and contribute to the water permeability of epithelial and endothelial cells. Currently 11 members of the AQP family (AQP0-10) have been reported in mammals. Here we report the identification of AQP12, which we found by performing a BLAST program search. Northern blot analysis revealed that AQP12 was specifically expressed in the pancreas. Further analysis by in situ hybridization and RT-PCR studies showed that AQP12 was selectively localized in the acinar cells of the pancreas. To analyze the cellular localization and function of AQP12, we expressed AQP12 in Xenopus oocytes and cultured mammalian cells. Immunocytochemistry revealed that AQP12 was not targeted to the plasma membrane. The selective localization of AQP12 in pancreatic acinar cells and possibly in the intracellular organelles suggests a role of AQP12 in digestive enzyme secretion such as maturation and exocytosis of secretory granules.

Original languageEnglish
Pages (from-to)832-838
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume330
Issue number3
DOIs
Publication statusPublished - 2005 May 13
Externally publishedYes

Fingerprint

Aquaporins
Acinar Cells
Pancreas
Mammals
Endothelial cells
Exocytosis
Secretory Vesicles
Cell membranes
Xenopus
Northern Blotting
Organelles
Oocytes
In Situ Hybridization
Cultured Cells
Permeability
Endothelial Cells
Epithelial Cells
Immunohistochemistry
Cells
Cell Membrane

Keywords

  • Acinar cell
  • AQP
  • Aquaporin
  • Pancreas
  • Water channel

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of a novel aquaporin, AQP12, expressed in pancreatic acinar cells. / Itoh, Tomohiro; Rai, Tatemitsu; Kuwahara, Michio; Ko, Shigeru; Uchida, Shinichi; Sasaki, Sei; Ishibashi, Kenichi.

In: Biochemical and Biophysical Research Communications, Vol. 330, No. 3, 13.05.2005, p. 832-838.

Research output: Contribution to journalArticle

Itoh, Tomohiro ; Rai, Tatemitsu ; Kuwahara, Michio ; Ko, Shigeru ; Uchida, Shinichi ; Sasaki, Sei ; Ishibashi, Kenichi. / Identification of a novel aquaporin, AQP12, expressed in pancreatic acinar cells. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 330, No. 3. pp. 832-838.
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