Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in human breast cancer cell lines

Koichi Nagasaki; Nicolai Maass; Tomohiro Manabe; Hiroaki Hanzawa; Toshihiko Tsukada; Kiyoshi Kikuchi; Ken Yamaguchi

doi:10.1016/S0304-3835(99)00091-9

Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in human breast cancer cell lines

Koichi Nagasaki, Nicolai Maass, Tomohiro Manabe, Hiroaki Hanzawa, Toshihiko Tsukada, Kiyoshi Kikuchi, Ken Yamaguchi

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Screening for differentially expressed genes in cancer cell lines by the RNA differential display (DD) technique identified a novel mRNA that encodes a 26-kDa protein and is up-regulated in MCF-7 and BT-20 breast cancer cells. The predicted amino acid sequence showed 38% homology to Caenorhabditis elegans T12A2.7 protein, indicating that this is a possible human homologue for C. elegans T12A2.7 protein. The mRNA, designated DAM1 (DNA amplified in mammary carcinoma), was mapped at the 1p13.3-21 region, which is frequently altered in human breast cancer. By Southern blot analysis, we confirmed that the up-regulation of this novel gene is associated with gene amplification in MCF-7 (10-fold) and BT-20 (5-fold) cells. Our findings suggest that the DAM1 gene is a novel gene up-regulated by amplification in human breast cancer cell lines. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	219-226
Number of pages	8
Journal	Cancer Letters
Volume	140
Issue number	1-2
DOIs	https://doi.org/10.1016/S0304-3835(99)00091-9
Publication status	Published - 1999 Jun 1
Externally published	Yes

Keywords

Amplification
Breast cancer
C. elegans
Chromosome 1
Differential display

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/S0304-3835(99)00091-9

Cite this

@article{d6c128ee2c5a4a84aa811be98a3027a2,

title = "Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in human breast cancer cell lines",

abstract = "Screening for differentially expressed genes in cancer cell lines by the RNA differential display (DD) technique identified a novel mRNA that encodes a 26-kDa protein and is up-regulated in MCF-7 and BT-20 breast cancer cells. The predicted amino acid sequence showed 38% homology to Caenorhabditis elegans T12A2.7 protein, indicating that this is a possible human homologue for C. elegans T12A2.7 protein. The mRNA, designated DAM1 (DNA amplified in mammary carcinoma), was mapped at the 1p13.3-21 region, which is frequently altered in human breast cancer. By Southern blot analysis, we confirmed that the up-regulation of this novel gene is associated with gene amplification in MCF-7 (10-fold) and BT-20 (5-fold) cells. Our findings suggest that the DAM1 gene is a novel gene up-regulated by amplification in human breast cancer cell lines. Copyright (C) 1999 Elsevier Science Ireland Ltd.",

keywords = "Amplification, Breast cancer, C. elegans, Chromosome 1, Differential display",

author = "Koichi Nagasaki and Nicolai Maass and Tomohiro Manabe and Hiroaki Hanzawa and Toshihiko Tsukada and Kiyoshi Kikuchi and Ken Yamaguchi",

note = "Funding Information: This work was supported in part by a grant-in-aid from the Ministry of Health and Welfare, Japan, for the second Term Comprehensive 10-Year Strategy for Cancer Control and for Cancer Research (9-32 and 10-28).",

year = "1999",

month = jun,

day = "1",

doi = "10.1016/S0304-3835(99)00091-9",

language = "English",

volume = "140",

pages = "219--226",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "1-2",

}

TY - JOUR

T1 - Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in human breast cancer cell lines

AU - Nagasaki, Koichi

AU - Maass, Nicolai

AU - Manabe, Tomohiro

AU - Hanzawa, Hiroaki

AU - Tsukada, Toshihiko

AU - Kikuchi, Kiyoshi

AU - Yamaguchi, Ken

N1 - Funding Information: This work was supported in part by a grant-in-aid from the Ministry of Health and Welfare, Japan, for the second Term Comprehensive 10-Year Strategy for Cancer Control and for Cancer Research (9-32 and 10-28).

PY - 1999/6/1

Y1 - 1999/6/1

N2 - Screening for differentially expressed genes in cancer cell lines by the RNA differential display (DD) technique identified a novel mRNA that encodes a 26-kDa protein and is up-regulated in MCF-7 and BT-20 breast cancer cells. The predicted amino acid sequence showed 38% homology to Caenorhabditis elegans T12A2.7 protein, indicating that this is a possible human homologue for C. elegans T12A2.7 protein. The mRNA, designated DAM1 (DNA amplified in mammary carcinoma), was mapped at the 1p13.3-21 region, which is frequently altered in human breast cancer. By Southern blot analysis, we confirmed that the up-regulation of this novel gene is associated with gene amplification in MCF-7 (10-fold) and BT-20 (5-fold) cells. Our findings suggest that the DAM1 gene is a novel gene up-regulated by amplification in human breast cancer cell lines. Copyright (C) 1999 Elsevier Science Ireland Ltd.

AB - Screening for differentially expressed genes in cancer cell lines by the RNA differential display (DD) technique identified a novel mRNA that encodes a 26-kDa protein and is up-regulated in MCF-7 and BT-20 breast cancer cells. The predicted amino acid sequence showed 38% homology to Caenorhabditis elegans T12A2.7 protein, indicating that this is a possible human homologue for C. elegans T12A2.7 protein. The mRNA, designated DAM1 (DNA amplified in mammary carcinoma), was mapped at the 1p13.3-21 region, which is frequently altered in human breast cancer. By Southern blot analysis, we confirmed that the up-regulation of this novel gene is associated with gene amplification in MCF-7 (10-fold) and BT-20 (5-fold) cells. Our findings suggest that the DAM1 gene is a novel gene up-regulated by amplification in human breast cancer cell lines. Copyright (C) 1999 Elsevier Science Ireland Ltd.

KW - Amplification

KW - Breast cancer

KW - C. elegans

KW - Chromosome 1

KW - Differential display

UR - http://www.scopus.com/inward/record.url?scp=0033014153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033014153&partnerID=8YFLogxK

U2 - 10.1016/S0304-3835(99)00091-9

DO - 10.1016/S0304-3835(99)00091-9

M3 - Article

C2 - 10403562

AN - SCOPUS:0033014153

SN - 0304-3835

VL - 140

SP - 219

EP - 226

JO - Cancer Letters

JF - Cancer Letters

IS - 1-2

ER -

Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in human breast cancer cell lines

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this