Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in human breast cancer cell lines

Koichi Nagasaki, Nicolai Maass, Tomohiro Manabe, Hiroaki Hanzawa, Toshihiko Tsukada, Kiyoshi Kikuchi, Ken Yamaguchi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Screening for differentially expressed genes in cancer cell lines by the RNA differential display (DD) technique identified a novel mRNA that encodes a 26-kDa protein and is up-regulated in MCF-7 and BT-20 breast cancer cells. The predicted amino acid sequence showed 38% homology to Caenorhabditis elegans T12A2.7 protein, indicating that this is a possible human homologue for C. elegans T12A2.7 protein. The mRNA, designated DAM1 (DNA amplified in mammary carcinoma), was mapped at the 1p13.3-21 region, which is frequently altered in human breast cancer. By Southern blot analysis, we confirmed that the up-regulation of this novel gene is associated with gene amplification in MCF-7 (10-fold) and BT-20 (5-fold) cells. Our findings suggest that the DAM1 gene is a novel gene up-regulated by amplification in human breast cancer cell lines. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)219-226
Number of pages8
JournalCancer Letters
Volume140
Issue number1-2
DOIs
Publication statusPublished - 1999 Jun 1
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 21
Caenorhabditis elegans Proteins
Breast Neoplasms
Cell Line
Genes
Messenger RNA
Gene Amplification
Neoplasm Genes
Southern Blotting
Amino Acid Sequence
Up-Regulation
RNA
DNA
Proteins

Keywords

  • Amplification
  • Breast cancer
  • C. elegans
  • Chromosome 1
  • Differential display

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in human breast cancer cell lines. / Nagasaki, Koichi; Maass, Nicolai; Manabe, Tomohiro; Hanzawa, Hiroaki; Tsukada, Toshihiko; Kikuchi, Kiyoshi; Yamaguchi, Ken.

In: Cancer Letters, Vol. 140, No. 1-2, 01.06.1999, p. 219-226.

Research output: Contribution to journalArticle

Nagasaki, Koichi ; Maass, Nicolai ; Manabe, Tomohiro ; Hanzawa, Hiroaki ; Tsukada, Toshihiko ; Kikuchi, Kiyoshi ; Yamaguchi, Ken. / Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in human breast cancer cell lines. In: Cancer Letters. 1999 ; Vol. 140, No. 1-2. pp. 219-226.
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