Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in human breast cancer cell lines

Koichi Nagasaki, Nicolai Maass, Tomohiro Manabe, Hiroaki Hanzawa, Toshihiko Tsukada, Kiyoshi Kikuchi, Ken Yamaguchi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Screening for differentially expressed genes in cancer cell lines by the RNA differential display (DD) technique identified a novel mRNA that encodes a 26-kDa protein and is up-regulated in MCF-7 and BT-20 breast cancer cells. The predicted amino acid sequence showed 38% homology to Caenorhabditis elegans T12A2.7 protein, indicating that this is a possible human homologue for C. elegans T12A2.7 protein. The mRNA, designated DAM1 (DNA amplified in mammary carcinoma), was mapped at the 1p13.3-21 region, which is frequently altered in human breast cancer. By Southern blot analysis, we confirmed that the up-regulation of this novel gene is associated with gene amplification in MCF-7 (10-fold) and BT-20 (5-fold) cells. Our findings suggest that the DAM1 gene is a novel gene up-regulated by amplification in human breast cancer cell lines. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)219-226
Number of pages8
JournalCancer Letters
Volume140
Issue number1-2
DOIs
Publication statusPublished - 1999 Jun 1
Externally publishedYes

Keywords

  • Amplification
  • Breast cancer
  • C. elegans
  • Chromosome 1
  • Differential display

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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