Identification of a novel gene, LDOC1, down-regulated in cancer cell lines

Koichi Nagasaki, Tomohiro Manabe, Hiroaki Hanzawa, Nicolai Maass, Toshihiko Tsukada, Ken Yamaguchi

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

By screening for differentially expressed genes in cancer cells, using the RNA differential display (DD) technique, we identified a novel cDNA, LDOC1, that is down-regulated in some cancer cell lines. A Northern blot analysis revealed no expression in pancreatic and gastric cancer cell lines but ubiquitous expression in normal human tissues. This new gene was mapped on chromosome Xq27 and the predicted protein sequence showed no similarity to known sequences in the database except for a leucine zipper-like motif at the N-terminal region and a proline-rich region that shares marked similarity to an SH3-binding domain. In an enhanced green fluorescent protein (EGFP) assay, the EGFP-LDOC1 fusion protein was localized in the nucleus. Although the function of LDOC1 is still unknown, our results suggest that this novel gene codes for a nuclear protein, and down-regulation of LDOC1 may have an important role in the development and/or progression of some cancers. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)227-234
Number of pages8
JournalCancer Letters
Volume140
Issue number1-2
DOIs
Publication statusPublished - 1999 Jun 1
Externally publishedYes

Keywords

  • Chromosome X
  • Leucine zipper
  • Nuclear protein
  • SH3-binding domain

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Nagasaki, K., Manabe, T., Hanzawa, H., Maass, N., Tsukada, T., & Yamaguchi, K. (1999). Identification of a novel gene, LDOC1, down-regulated in cancer cell lines. Cancer Letters, 140(1-2), 227-234. https://doi.org/10.1016/S0304-3835(99)00087-7