Identification of a novel, tissue-specific calpain htra-3; a human homologue of the Caenorhabditis elegans sex determination gene

Norifumi Mugita, Yoriyoshi Kimura, Michio Ogawa, Hideyuki Saya, Mitsuyoshi Nakao

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We cloned a novel human gene encoding a tissue-specific calpain, termed htra-3, which is highly homologous to the tra-3 sex determination gene of Caenorhabditis elegans. The predicted htra-3 polypeptide had similarity to the calpain large subunits in domain organization throughout domains I to III, but the sequences of domain IV lacked calcium-binding motifs. Northern blot analysis revealed high expression in the colon, small intestine and testis. Radiation hybrid mapping localized the htra-3 gene to chromosome 11q14 (2.53cR apart from WI-3895). Western blot analysis demonstrated that the approximately 73-kDa htra-3 protein was transiently expressed in COS-7 cells. These observations, together with the genetic information in C. elegans, suggest a unique function for htra-3 protein.

Original languageEnglish
Pages (from-to)845-850
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume239
Issue number3
DOIs
Publication statusPublished - 1997 Oct 29
Externally publishedYes

Fingerprint

Calpain
Caenorhabditis elegans
Genes
Tissue
Gene encoding
Chromosomes
Radiation Hybrid Mapping
Proteins
COS Cells
Calcium
Radiation
Northern Blotting
Peptides
Small Intestine
Testis
Colon
Western Blotting

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of a novel, tissue-specific calpain htra-3; a human homologue of the Caenorhabditis elegans sex determination gene. / Mugita, Norifumi; Kimura, Yoriyoshi; Ogawa, Michio; Saya, Hideyuki; Nakao, Mitsuyoshi.

In: Biochemical and Biophysical Research Communications, Vol. 239, No. 3, 29.10.1997, p. 845-850.

Research output: Contribution to journalArticle

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