Identification of a novel zinc-binding protein, C1orf123, as an interactor with a heavy metalassociated domain

Yoshiaki Furukawa, Carolyn Lim, Takehiko Tosha, Koki Yoshida, Tomoaki Hagai, Shuji Akiyama, Shoji Watanabe, Kenta Nakagome, Yoshitsugu Shiro

Research output: Contribution to journalArticle

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Abstract

Heavy metal-associated (HMA) domains bind metal ions at its Cys-x-x-Cys (CxxC) motif and constitute an intracellular network for trafficking of metal ions for utilization and detoxification. We thus expect that novel metalloproteins can be identified by screening proteins interacting with a HMA domain. In this study, we performed yeast two-hybrid screening of the human proteome and found an uncharacterized protein encoded as open reading frame 123 in chromosome 1 (C1orf123) that can interact specifically with the HMA domain of a copper chaperone for superoxide dismutase (CCSdI). Our X-ray structural analysis of C1orf123 further revealed that it binds a Zn2+ ion in a tetrahedral coordination with four thiolate groups from two conserved CxxC motifs. For the interaction between C1orf123 and CCSdI, the CxxC motifs in both C1orf123 and CCSdI were required, implying metal-mediated interaction through the CxxC motifs. Notably, C1orf123 did not interact with several other HMA domains containing CxxC motifs, supporting high specificity in the interaction between C1orf123 and CCSdI. Based upon these results, we further discuss functional and structural significance of the interaction between C1orf123 and CCS.

Original languageEnglish
Article numbere0204355
JournalPLoS One
Volume13
Issue number9
DOIs
Publication statusPublished - 2018 Sep 1

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Heavy Metals
Superoxide Dismutase
binding proteins
Copper
superoxide dismutase
heavy metals
copper
zinc
Metals
Ions
metal ions
Metal ions
Screening
metalloproteins
Metalloproteins
two hybrid system techniques
Detoxification
Chromosomes, Human, Pair 1
Proteome
Chromosomes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Identification of a novel zinc-binding protein, C1orf123, as an interactor with a heavy metalassociated domain. / Furukawa, Yoshiaki; Lim, Carolyn; Tosha, Takehiko; Yoshida, Koki; Hagai, Tomoaki; Akiyama, Shuji; Watanabe, Shoji; Nakagome, Kenta; Shiro, Yoshitsugu.

In: PLoS One, Vol. 13, No. 9, e0204355, 01.09.2018.

Research output: Contribution to journalArticle

Furukawa, Y, Lim, C, Tosha, T, Yoshida, K, Hagai, T, Akiyama, S, Watanabe, S, Nakagome, K & Shiro, Y 2018, 'Identification of a novel zinc-binding protein, C1orf123, as an interactor with a heavy metalassociated domain', PLoS One, vol. 13, no. 9, e0204355. https://doi.org/10.1371/journal.pone.0204355
Furukawa, Yoshiaki ; Lim, Carolyn ; Tosha, Takehiko ; Yoshida, Koki ; Hagai, Tomoaki ; Akiyama, Shuji ; Watanabe, Shoji ; Nakagome, Kenta ; Shiro, Yoshitsugu. / Identification of a novel zinc-binding protein, C1orf123, as an interactor with a heavy metalassociated domain. In: PLoS One. 2018 ; Vol. 13, No. 9.
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