Identification of a pathogenic isolate-specific 30,000-M(r) antigen of Entamoeba histolytica by using a monoclonal antibody

H. Tachibana, S. Kobayashi, Y. Kato, K. Nagakura, Y. Kaneda, T. Takeuchi

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

A monoclonal antibody (MAb) produced against trophozoites of Entamoeba histolytica strain HM-1:IMSS, reacted with all of 42 isolates and 4 clones showing pathogenic zymodeme (Z) patterns, i.e., Z-II, Z-IIα-, Z-II (glucose phosphate isomerase:γ+), Z-VII, Z-VII (glucose phosphate isomerase:α lack, γ+), Z-XI, Z-XIV, and Z-XIX, regardless of culture conditions, geographical origins, or host symptoms in an indirect fluorescence antibody test. In contrast, the MAb failed to react with 14 isolates possessing nonpathogenic zymodemes Z-I and Z-VIII and did not react with other enteric protozoan parasites, such as E. histolytica-like Laredo, Entamoeba hartmanni, Entamoeba coli, Endolimax nana, Dientamoeba fragilis, Trichomonas hominis, and Giardia lamblia. Western immunoblotting analysis showed that the molecular weight of the antigenic component recognized by the MAb was exclusively 30,000 in pathogenic isolates of different zymodemes. These results suggest that the 30,000-molecular-weight antigen is a marker of pathogenic isolates and that the indirect fluorescent-antibody test with the MAb is useful for the accurate discrimination of pathogenic amebae.

Original languageEnglish
Pages (from-to)955-960
Number of pages6
JournalInfection and Immunity
Volume58
Issue number4
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Entamoeba histolytica
xylose isomerase
Monoclonal Antibodies
Entamoeba
Antigens
Dientamoeba
Endolimax
Molecular Weight
Phosphates
Trichomonas
Giardia lamblia
Trophozoites
Amoeba
Antibodies
Parasites
Clone Cells
Fluorescence
Western Blotting

ASJC Scopus subject areas

  • Immunology

Cite this

Tachibana, H., Kobayashi, S., Kato, Y., Nagakura, K., Kaneda, Y., & Takeuchi, T. (1990). Identification of a pathogenic isolate-specific 30,000-M(r) antigen of Entamoeba histolytica by using a monoclonal antibody. Infection and Immunity, 58(4), 955-960.

Identification of a pathogenic isolate-specific 30,000-M(r) antigen of Entamoeba histolytica by using a monoclonal antibody. / Tachibana, H.; Kobayashi, S.; Kato, Y.; Nagakura, K.; Kaneda, Y.; Takeuchi, T.

In: Infection and Immunity, Vol. 58, No. 4, 1990, p. 955-960.

Research output: Contribution to journalArticle

Tachibana, H, Kobayashi, S, Kato, Y, Nagakura, K, Kaneda, Y & Takeuchi, T 1990, 'Identification of a pathogenic isolate-specific 30,000-M(r) antigen of Entamoeba histolytica by using a monoclonal antibody', Infection and Immunity, vol. 58, no. 4, pp. 955-960.
Tachibana, H. ; Kobayashi, S. ; Kato, Y. ; Nagakura, K. ; Kaneda, Y. ; Takeuchi, T. / Identification of a pathogenic isolate-specific 30,000-M(r) antigen of Entamoeba histolytica by using a monoclonal antibody. In: Infection and Immunity. 1990 ; Vol. 58, No. 4. pp. 955-960.
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