Identification of a platelet-aggregating factor of murine colon adenocarcinoma 26: M(r) 44,000 membrane protein as determined by monoclonal antibodies

M. Watanabe, E. Okochi, Yoshikazu Sugimoto, T. Tsuruo

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Abstract

The interaction between platelets and tumor cells plays an important role in the hematogenous spread of certain malignant cancers. We found that metastatic clones of murine colon adenocarcinoma 26 induced platelet aggregation in a membrane protein-dependent manner. Two monoclonal antibodies (mAbs) of IgG(2a) class were generated against a highly metastatic colon 26 clone, NL-17. These two mAbs, designated 8F11 and 20A11, showed a two-fold higher level of NL-17 binding than a low metastatic clone, NL-14, which possesses low platelet-aggregating ability. Both mAbs retarded platelet aggregation induced by NL-17. Western blot analysis showed that both mAbs recognized the same M(r) 44,000 membrane protein as antigen under reducing conditions. Trypsin treatment of NL-17 diminished the ability of the cells to induce platelet-aggregation and resulted in a decrease in the reactivity of the cells to 8F11. These results suggest that the M(r) 44,000 membrane protein recognized by the two mAbs is a platelet-aggregating factor of colon 26 cells.

Original languageEnglish
Pages (from-to)6411-6416
Number of pages6
JournalCancer Research
Volume48
Issue number22
Publication statusPublished - 1988
Externally publishedYes

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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