Identification of a WT1 protein-derived peptide, WT1187, as a HLA-A*0206-restricted, WT1-specific CTL epitope

Zheyu Li, Yoshihiro Oka, Akihiro Tsuboi, Fumihiro Fujiki, Yukie Harada, Hiroko Nakajima, Tomoki Masuda, Yoko Fukuda, Mai Kawakatsu, Soyoko Morimoto, Takamasa Katagiri, Naoya Tatsumi, Naoki Hosen, Toshiaki Shirakata, Sumiyuki Nishida, Yutaka Kawakami, Keiko Udaka, Ichiro Kawase, Yusuke Oji, Haruo Sugiyama

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Abstract

The Wilms' tumor gene WT1 is overexpressed in various kinds of hematopoietic malignancies as well as solid cancers, and this protein has been demonstrated to be an attractive target antigen for cancer immunotherapy. WT1-specific CTL epitopeswith a restriction of HLA-A*2402 or HLA-A*0201 have been already identified. In the present study it has been demonstrated that a 9-mer WT1-derived WT1187 peptide, which had already been shown to elicit a WT1-specific CTL response with a restriction of HLA-A*0201, can also elicit a CTL response with a restriction of HLA-A*0206. In all three different HLA-A*0206+ healthy donors examined, WT1187 peptide-specific CTL could be generated from peripheral blood mononuclear cells, and the CTL showed cytotoxic activity that depended on dual expression of WT1 and HLA-A*0206 molecules. The present study describes the first identification of a HLA-A*0206-restricted, WT1-specific CTL epitope. The present results should help to broaden the application of WT1 peptide-based immunotherapy fromonly HLA-A*0201- positive to HLA-A*0206-positive cancer patients as well.

Original languageEnglish
Pages (from-to)551-558
Number of pages8
JournalMicrobiology and Immunology
Volume52
Issue number11
DOIs
Publication statusPublished - 2008 Nov 1

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Keywords

  • Cancer
  • HLA-A*0206
  • Vaccine
  • WT1

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this

Li, Z., Oka, Y., Tsuboi, A., Fujiki, F., Harada, Y., Nakajima, H., Masuda, T., Fukuda, Y., Kawakatsu, M., Morimoto, S., Katagiri, T., Tatsumi, N., Hosen, N., Shirakata, T., Nishida, S., Kawakami, Y., Udaka, K., Kawase, I., Oji, Y., & Sugiyama, H. (2008). Identification of a WT1 protein-derived peptide, WT1187, as a HLA-A*0206-restricted, WT1-specific CTL epitope. Microbiology and Immunology, 52(11), 551-558. https://doi.org/10.1111/j.1348-0421.2008.00069.x