Identification of Actin as a Substrate of ICE and an ICE-like Protease and Involvement of an ICE-like Protease but Not ICE in Vp-16-Induced U937 Apoptosis

T. Mashima, M. Naito, N. Fujita, Kohji Noguchi, T. Tsuruo

Research output: Contribution to journalArticle

196 Citations (Scopus)

Abstract

Human leukemia U937 cells are induced to undergo apoptosis by several chemotherapeutic agents; however, the cellular components involved in the process have not yet been identified. We found that an actin-cleavage activity (ACA) was activated in the VP-16-treated U937 cytosolic fraction and 15K- and 30K-actin fragments were produced. This ACA was inhibited by inhibitors of interleukin-1β-converting enzyme (ICE)/ced-3 family proteases, such as Z-Asp-CH2-DCB, YVAD-CHO, TPCK, TLCK, and iodoacetamide. Differing from ICE, the ACA could not process pro-IL-1β to mature IL-1β. Although ICE can cleave actin in vitro, ICE activity was not activated in the VP-16 treated U937 cells. These results indicate that actin is a potential substrate of ICE and ICE-like proteases, and that VP-16 preferentially activate an ICE-like protease, but not ICE itself, in U937 cells.

Original languageEnglish
Pages (from-to)1185-1192
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume217
Issue number3
DOIs
Publication statusPublished - 1995 Dec 26
Externally publishedYes

Fingerprint

Caspase 1
Actins
Peptide Hydrolases
Apoptosis
Substrates
U937 Cells
Etoposide
Interleukin-1
Tosyllysine Chloromethyl Ketone
Tosylphenylalanyl Chloromethyl Ketone
Iodoacetamide
Enzyme activity
Leukemia

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

Cite this

Identification of Actin as a Substrate of ICE and an ICE-like Protease and Involvement of an ICE-like Protease but Not ICE in Vp-16-Induced U937 Apoptosis. / Mashima, T.; Naito, M.; Fujita, N.; Noguchi, Kohji; Tsuruo, T.

In: Biochemical and Biophysical Research Communications, Vol. 217, No. 3, 26.12.1995, p. 1185-1192.

Research output: Contribution to journalArticle

@article{b491bf8ebec1468fb34551d82f18f77f,
title = "Identification of Actin as a Substrate of ICE and an ICE-like Protease and Involvement of an ICE-like Protease but Not ICE in Vp-16-Induced U937 Apoptosis",
abstract = "Human leukemia U937 cells are induced to undergo apoptosis by several chemotherapeutic agents; however, the cellular components involved in the process have not yet been identified. We found that an actin-cleavage activity (ACA) was activated in the VP-16-treated U937 cytosolic fraction and 15K- and 30K-actin fragments were produced. This ACA was inhibited by inhibitors of interleukin-1β-converting enzyme (ICE)/ced-3 family proteases, such as Z-Asp-CH2-DCB, YVAD-CHO, TPCK, TLCK, and iodoacetamide. Differing from ICE, the ACA could not process pro-IL-1β to mature IL-1β. Although ICE can cleave actin in vitro, ICE activity was not activated in the VP-16 treated U937 cells. These results indicate that actin is a potential substrate of ICE and ICE-like proteases, and that VP-16 preferentially activate an ICE-like protease, but not ICE itself, in U937 cells.",
author = "T. Mashima and M. Naito and N. Fujita and Kohji Noguchi and T. Tsuruo",
year = "1995",
month = "12",
day = "26",
doi = "10.1006/bbrc.1995.2894",
language = "English",
volume = "217",
pages = "1185--1192",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Identification of Actin as a Substrate of ICE and an ICE-like Protease and Involvement of an ICE-like Protease but Not ICE in Vp-16-Induced U937 Apoptosis

AU - Mashima, T.

AU - Naito, M.

AU - Fujita, N.

AU - Noguchi, Kohji

AU - Tsuruo, T.

PY - 1995/12/26

Y1 - 1995/12/26

N2 - Human leukemia U937 cells are induced to undergo apoptosis by several chemotherapeutic agents; however, the cellular components involved in the process have not yet been identified. We found that an actin-cleavage activity (ACA) was activated in the VP-16-treated U937 cytosolic fraction and 15K- and 30K-actin fragments were produced. This ACA was inhibited by inhibitors of interleukin-1β-converting enzyme (ICE)/ced-3 family proteases, such as Z-Asp-CH2-DCB, YVAD-CHO, TPCK, TLCK, and iodoacetamide. Differing from ICE, the ACA could not process pro-IL-1β to mature IL-1β. Although ICE can cleave actin in vitro, ICE activity was not activated in the VP-16 treated U937 cells. These results indicate that actin is a potential substrate of ICE and ICE-like proteases, and that VP-16 preferentially activate an ICE-like protease, but not ICE itself, in U937 cells.

AB - Human leukemia U937 cells are induced to undergo apoptosis by several chemotherapeutic agents; however, the cellular components involved in the process have not yet been identified. We found that an actin-cleavage activity (ACA) was activated in the VP-16-treated U937 cytosolic fraction and 15K- and 30K-actin fragments were produced. This ACA was inhibited by inhibitors of interleukin-1β-converting enzyme (ICE)/ced-3 family proteases, such as Z-Asp-CH2-DCB, YVAD-CHO, TPCK, TLCK, and iodoacetamide. Differing from ICE, the ACA could not process pro-IL-1β to mature IL-1β. Although ICE can cleave actin in vitro, ICE activity was not activated in the VP-16 treated U937 cells. These results indicate that actin is a potential substrate of ICE and ICE-like proteases, and that VP-16 preferentially activate an ICE-like protease, but not ICE itself, in U937 cells.

UR - http://www.scopus.com/inward/record.url?scp=0029610432&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029610432&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1995.2894

DO - 10.1006/bbrc.1995.2894

M3 - Article

C2 - 8554575

AN - SCOPUS:0029610432

VL - 217

SP - 1185

EP - 1192

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -