Identification of ADAMTS13 peptide sequences binding to von Willebrand factor

Takanori Moriki, Ichiro N. Maruyama, Atsuko Igari, Yasuo Ikeda, Mitsuru Murata

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

ADAMTS13 cleaves multimeric von Willebrand factor (VWF) to regulate VWF-mediated thrombus formation. To search ADAMTS13 peptide sequences binding to VWF, a λ-phage library expressing various peptides of ADAMTS13 on the surface was screened using VWF either immobilized or in solution under static condition. By the first screening, peptides sharing the C-terminus of spacer domain from Arg670 to Gln684 (epitope-A) were selected. To explore additional sites, peptide sequences from the first screening were synthesized and added to the second screening. Consequently, Pro618 to Glu641 (epitope-B) in the middle of spacer domain was obtained from immobilized VWF condition. Synthetic epitope-B peptide inhibited the cleavage of VWF by ADAMTS13, while the synthetic epitope-A peptide did not as efficiently as epitope-B. Elimination of four amino acids from either sides of epitope-B terminus markedly reduced the inhibitory effect. These two sites in the spacer domain may play significant roles in binding to VWF.

Original languageEnglish
Pages (from-to)783-788
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume391
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1

Fingerprint

von Willebrand Factor
Epitopes
Peptides
Screening
Bacteriophages
Thrombosis
Amino Acids

Keywords

  • ADAMTS13
  • Phage display
  • TTP
  • VWF

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Identification of ADAMTS13 peptide sequences binding to von Willebrand factor. / Moriki, Takanori; Maruyama, Ichiro N.; Igari, Atsuko; Ikeda, Yasuo; Murata, Mitsuru.

In: Biochemical and Biophysical Research Communications, Vol. 391, No. 1, 01.01.2010, p. 783-788.

Research output: Contribution to journalArticle

Moriki, Takanori ; Maruyama, Ichiro N. ; Igari, Atsuko ; Ikeda, Yasuo ; Murata, Mitsuru. / Identification of ADAMTS13 peptide sequences binding to von Willebrand factor. In: Biochemical and Biophysical Research Communications. 2010 ; Vol. 391, No. 1. pp. 783-788.
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