Identification of biological functions and gene networks regulated by heat stress in U937 human lymphoma cells

Yukihiro Furusawa, Yoshiaki Tabuchi, Shigehito Wada, Ichiro Takasaki, Kenzo Ohtsuka, Takashi Kondo

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Although cancer cells exposed to temperatures >42.5°C undergo cell death as the temperature rises, exposure of up to 42.5°C induces slight orno cytotoxicity. The temperature of 42.5°C is, therefore, well known to be the inflection point of hyperthermia. To better understand the molecular mechanisms underlying cellular responses to heat stress at temperatures higher and lower than the inflection point, we carried out global scale microarray and computational gene expression analyses. Human leukemia U937 cells were incubated at 42°C or 44°C for 15 min and cultured at 37°C for 0-6 h. Apoptosis accompanied by the activation of caspase-3 and DNA fragmentation was only observed in cells treated with heat stress at 44°C, but not at 42°C. Although a large number of genes were differentially expressed by a factor of 2.0 or greater, we found substantial differences with respect to the biological functions and gene networks of the genes differentially expressed at the two temperatures examined. Interestingly, we identified temperature-specific gene networks that were considered to be mainly associated with cell death or cellular compromise and cellular function and maintenance at 44°C or 42°C, respectively, by using the Ingenuity pathway analysis tools. These findings provide the molecular basis for a further understanding of the mechanisms of the biological changes that are responsive to heat stress in human lymphoma cells.

Original languageEnglish
Pages (from-to)143-151
Number of pages9
JournalInternational Journal of Molecular Medicine
Volume28
Issue number2
DOIs
Publication statusPublished - 2011 Aug

Fingerprint

Gene Regulatory Networks
Lymphoma
Hot Temperature
Temperature
Cell Death
Heat-Shock Response
U937 Cells
DNA Fragmentation
Caspase 3
Genes
Leukemia
Fever
Maintenance
Apoptosis
Gene Expression
Neoplasms

Keywords

  • Apoptosis
  • Gene function
  • Gene network
  • Heat stress
  • Microarray

ASJC Scopus subject areas

  • Genetics

Cite this

Identification of biological functions and gene networks regulated by heat stress in U937 human lymphoma cells. / Furusawa, Yukihiro; Tabuchi, Yoshiaki; Wada, Shigehito; Takasaki, Ichiro; Ohtsuka, Kenzo; Kondo, Takashi.

In: International Journal of Molecular Medicine, Vol. 28, No. 2, 08.2011, p. 143-151.

Research output: Contribution to journalArticle

Furusawa, Yukihiro ; Tabuchi, Yoshiaki ; Wada, Shigehito ; Takasaki, Ichiro ; Ohtsuka, Kenzo ; Kondo, Takashi. / Identification of biological functions and gene networks regulated by heat stress in U937 human lymphoma cells. In: International Journal of Molecular Medicine. 2011 ; Vol. 28, No. 2. pp. 143-151.
@article{9fd1e0b24d474bfc8e14158acd0ba28b,
title = "Identification of biological functions and gene networks regulated by heat stress in U937 human lymphoma cells",
abstract = "Although cancer cells exposed to temperatures >42.5°C undergo cell death as the temperature rises, exposure of up to 42.5°C induces slight orno cytotoxicity. The temperature of 42.5°C is, therefore, well known to be the inflection point of hyperthermia. To better understand the molecular mechanisms underlying cellular responses to heat stress at temperatures higher and lower than the inflection point, we carried out global scale microarray and computational gene expression analyses. Human leukemia U937 cells were incubated at 42°C or 44°C for 15 min and cultured at 37°C for 0-6 h. Apoptosis accompanied by the activation of caspase-3 and DNA fragmentation was only observed in cells treated with heat stress at 44°C, but not at 42°C. Although a large number of genes were differentially expressed by a factor of 2.0 or greater, we found substantial differences with respect to the biological functions and gene networks of the genes differentially expressed at the two temperatures examined. Interestingly, we identified temperature-specific gene networks that were considered to be mainly associated with cell death or cellular compromise and cellular function and maintenance at 44°C or 42°C, respectively, by using the Ingenuity pathway analysis tools. These findings provide the molecular basis for a further understanding of the mechanisms of the biological changes that are responsive to heat stress in human lymphoma cells.",
keywords = "Apoptosis, Gene function, Gene network, Heat stress, Microarray",
author = "Yukihiro Furusawa and Yoshiaki Tabuchi and Shigehito Wada and Ichiro Takasaki and Kenzo Ohtsuka and Takashi Kondo",
year = "2011",
month = "8",
doi = "10.3892/ijmm.2011.702",
language = "English",
volume = "28",
pages = "143--151",
journal = "International Journal of Molecular Medicine",
issn = "1107-3756",
publisher = "Spandidos Publications",
number = "2",

}

TY - JOUR

T1 - Identification of biological functions and gene networks regulated by heat stress in U937 human lymphoma cells

AU - Furusawa, Yukihiro

AU - Tabuchi, Yoshiaki

AU - Wada, Shigehito

AU - Takasaki, Ichiro

AU - Ohtsuka, Kenzo

AU - Kondo, Takashi

PY - 2011/8

Y1 - 2011/8

N2 - Although cancer cells exposed to temperatures >42.5°C undergo cell death as the temperature rises, exposure of up to 42.5°C induces slight orno cytotoxicity. The temperature of 42.5°C is, therefore, well known to be the inflection point of hyperthermia. To better understand the molecular mechanisms underlying cellular responses to heat stress at temperatures higher and lower than the inflection point, we carried out global scale microarray and computational gene expression analyses. Human leukemia U937 cells were incubated at 42°C or 44°C for 15 min and cultured at 37°C for 0-6 h. Apoptosis accompanied by the activation of caspase-3 and DNA fragmentation was only observed in cells treated with heat stress at 44°C, but not at 42°C. Although a large number of genes were differentially expressed by a factor of 2.0 or greater, we found substantial differences with respect to the biological functions and gene networks of the genes differentially expressed at the two temperatures examined. Interestingly, we identified temperature-specific gene networks that were considered to be mainly associated with cell death or cellular compromise and cellular function and maintenance at 44°C or 42°C, respectively, by using the Ingenuity pathway analysis tools. These findings provide the molecular basis for a further understanding of the mechanisms of the biological changes that are responsive to heat stress in human lymphoma cells.

AB - Although cancer cells exposed to temperatures >42.5°C undergo cell death as the temperature rises, exposure of up to 42.5°C induces slight orno cytotoxicity. The temperature of 42.5°C is, therefore, well known to be the inflection point of hyperthermia. To better understand the molecular mechanisms underlying cellular responses to heat stress at temperatures higher and lower than the inflection point, we carried out global scale microarray and computational gene expression analyses. Human leukemia U937 cells were incubated at 42°C or 44°C for 15 min and cultured at 37°C for 0-6 h. Apoptosis accompanied by the activation of caspase-3 and DNA fragmentation was only observed in cells treated with heat stress at 44°C, but not at 42°C. Although a large number of genes were differentially expressed by a factor of 2.0 or greater, we found substantial differences with respect to the biological functions and gene networks of the genes differentially expressed at the two temperatures examined. Interestingly, we identified temperature-specific gene networks that were considered to be mainly associated with cell death or cellular compromise and cellular function and maintenance at 44°C or 42°C, respectively, by using the Ingenuity pathway analysis tools. These findings provide the molecular basis for a further understanding of the mechanisms of the biological changes that are responsive to heat stress in human lymphoma cells.

KW - Apoptosis

KW - Gene function

KW - Gene network

KW - Heat stress

KW - Microarray

UR - http://www.scopus.com/inward/record.url?scp=79958166046&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79958166046&partnerID=8YFLogxK

U2 - 10.3892/ijmm.2011.702

DO - 10.3892/ijmm.2011.702

M3 - Article

VL - 28

SP - 143

EP - 151

JO - International Journal of Molecular Medicine

JF - International Journal of Molecular Medicine

SN - 1107-3756

IS - 2

ER -