Identification of bladder cancer antigens recognized by IgG antibodies of a patient with metastatic bladder cancer

Keiichi Ito, Tomonobu Fujita, Masanori Akada, Yukiko Kiniwa, Makoto Tsukamoto, Aiko Yamamoto, Yuriko Matsuzaki, Maiko Matsushita, Takako Asano, Jun Nakashima, Masaaki Tachibana, Masamichi Hayakawa, Hideyuki Ikeda, Masaru Murai, Yutaka Kawakami

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To identify tumor antigens useful for the diagnosis and treatment of patients with bladder cancer, a lambda phage cDNA library constructed from a high-grade bladder cancer cell line was screened with autologous serum from a patient with metastatic bladder cancer. Forty-eight distinct antigens were isolated. By evaluating the immunogenicity and the tissue-specific expression, KU-BL-1 and KU-BL-2 were identified as immunogenic antigens with restricted tissue expression. KU-BL-1 was found to be a putative human lipoic acid synthetase with a metal-binding site, CXXXCXXC, that was expressed in bladder cancer cell lines and most bladder cancer tissues, as well as normal bladder mucosa and testis tissues. Immunoglobulin (Ig)G antibody to KU-BL-1 was detected in 2 of 28 patients with bladder cancer, but not in 30 healthy individuals. KU-BL-2 was found to be a putative human kelch-like protein that was homologous to Drosophila kelch, with a BTB/POZ domain and kelch repeats. KU-BL-2 was expressed in bladder cancer cell lines, most bladder cancer tissues, testis and heart, but not in normal bladder mucosa. IgG antibody to KU-BL-2 was detected in 8 of 28 patients with bladder cancer, but not in 16 healthy individuals. Tumor reactive T cells were induced from peripheral blood mononuclear cells (PBMC) by stimulation with one of the HLA-A24 binding KU-BL-2 peptides. Therefore, KU-BL-1 and KU-BL-2, which showed preferential expression in bladder cancer with restricted expression in normal tissues, as well as immunogenicity in multiple patients with bladder cancer, may be useful for the development of diagnostic and therapeutic methods for patients with bladder cancer.

Original languageEnglish
Pages (from-to)712-724
Number of pages13
JournalInternational Journal of Cancer
Volume108
Issue number5
DOIs
Publication statusPublished - 2004 Feb 20

Fingerprint

Urinary Bladder Neoplasms
Immunoglobulin G
Antigens
Antibodies
Cell Line
Mucous Membrane
Urinary Bladder
HLA-A24 Antigen
Bacteriophage lambda
Thioctic Acid
Testicular Neoplasms
Neoplasm Antigens
Ligases
Gene Library
Drosophila
Testis
Blood Cells
Metals
Binding Sites
T-Lymphocytes

Keywords

  • Bladder cancer antigen
  • Kelch-like protein
  • Lipoic acid synthetase
  • SEREX
  • Tumor reactive T cell

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ito, K., Fujita, T., Akada, M., Kiniwa, Y., Tsukamoto, M., Yamamoto, A., ... Kawakami, Y. (2004). Identification of bladder cancer antigens recognized by IgG antibodies of a patient with metastatic bladder cancer. International Journal of Cancer, 108(5), 712-724. https://doi.org/10.1002/ijc.11625

Identification of bladder cancer antigens recognized by IgG antibodies of a patient with metastatic bladder cancer. / Ito, Keiichi; Fujita, Tomonobu; Akada, Masanori; Kiniwa, Yukiko; Tsukamoto, Makoto; Yamamoto, Aiko; Matsuzaki, Yuriko; Matsushita, Maiko; Asano, Takako; Nakashima, Jun; Tachibana, Masaaki; Hayakawa, Masamichi; Ikeda, Hideyuki; Murai, Masaru; Kawakami, Yutaka.

In: International Journal of Cancer, Vol. 108, No. 5, 20.02.2004, p. 712-724.

Research output: Contribution to journalArticle

Ito, K, Fujita, T, Akada, M, Kiniwa, Y, Tsukamoto, M, Yamamoto, A, Matsuzaki, Y, Matsushita, M, Asano, T, Nakashima, J, Tachibana, M, Hayakawa, M, Ikeda, H, Murai, M & Kawakami, Y 2004, 'Identification of bladder cancer antigens recognized by IgG antibodies of a patient with metastatic bladder cancer', International Journal of Cancer, vol. 108, no. 5, pp. 712-724. https://doi.org/10.1002/ijc.11625
Ito, Keiichi ; Fujita, Tomonobu ; Akada, Masanori ; Kiniwa, Yukiko ; Tsukamoto, Makoto ; Yamamoto, Aiko ; Matsuzaki, Yuriko ; Matsushita, Maiko ; Asano, Takako ; Nakashima, Jun ; Tachibana, Masaaki ; Hayakawa, Masamichi ; Ikeda, Hideyuki ; Murai, Masaru ; Kawakami, Yutaka. / Identification of bladder cancer antigens recognized by IgG antibodies of a patient with metastatic bladder cancer. In: International Journal of Cancer. 2004 ; Vol. 108, No. 5. pp. 712-724.
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