Identification of candidate genes involved in endogenous protection mechanisms against acute pancreatitis in mice

Shinji Nakada, Koichi Tsuneyama, Ichiro Kato, Yoshiaki Tabuchi, Ichiro Takasaki, Yukihiro Furusawa, Hiroshi Kawaguchi, Makoto Fujimoto, Hirozo Goto, Hiroaki Hikiami, Takashi Kondo, Yasuo Takano, Yutaka Shimada

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We surveyed changes of the gene expression profile in caerulein-exposed pancreas using Affymetrix GeneChip system (39,000 genes). Up-regulation of genes coding for claudin 4, claudin 7, F11 receptor, cadherin 1, integrin beta 4, syndecan 1, heat shock proteins b1/90aa1, Serpinb6a, Serpinb6b, Serpinb9, Bax, Bak1, calpain 2, calpain 5, microtubule-associated protein 1 light chain 3 alpha, S100 calcium-binding proteins A4/A10 were found in mouse pancreas exposed to caerulein for 12 h. In contrast, the anti-apoptotic gene Bcl2 was down-regulated. The functions of these genes concern tight junction formation, cell-cell/cell-matrix adhesions, stress response, protease inhibition, apoptosis, autophagy, and regulation of cytoskeletal dynamics. Caerulein-exposed pancreatic acinar cells were immunohistochemically stained for claudin 4, cadherin 1, integrin beta 4, heat shock protein b1, and Serpinb6a. In conclusion, we have newly identified a set of genes that are likely to be involved in endogenous self-protection mechanisms against acute pancreatitis.

Original languageEnglish
Pages (from-to)1342-1347
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume391
Issue number3
DOIs
Publication statusPublished - 2010 Jan 15

Keywords

  • Caerulein
  • Claudin
  • GeneChip
  • Heat shock protein
  • Pancreatitis
  • Protease inhibitor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Nakada, S., Tsuneyama, K., Kato, I., Tabuchi, Y., Takasaki, I., Furusawa, Y., Kawaguchi, H., Fujimoto, M., Goto, H., Hikiami, H., Kondo, T., Takano, Y., & Shimada, Y. (2010). Identification of candidate genes involved in endogenous protection mechanisms against acute pancreatitis in mice. Biochemical and Biophysical Research Communications, 391(3), 1342-1347. https://doi.org/10.1016/j.bbrc.2009.12.047