Identification of DNA topoisomerases involved in immediate and transient DNA relaxation induced by heat shock in Escherichia coli

Yasuyuki Ogata; Tohru Mizushima; Kazuhiro Kataoka; Takeyoshi Miki; Kazuhisa Sekimizu

doi:10.1007/BF00583895

Identification of DNA topoisomerases involved in immediate and transient DNA relaxation induced by heat shock in Escherichia coli

Yasuyuki Ogata, Tohru Mizushima, Kazuhiro Kataoka, Takeyoshi Miki, Kazuhisa Sekimizu

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The linking number of plasmid DNA in exponentially growing Escherichia coli increases immediately and transiently after heat shock. The purpose of this study was to search for DNA topoisomerases that catalyze this relaxation of DNA. Neither introduction of a topA deletion mutation nor treatment of cells with DNA gyrase inhibitors affected the DNA relaxation induced by heat shock. Thus, DNA topoisomerase I and DNA gyrase are apparently not involved in the process. However, the reaction was inhibited by nalidixic acid or by oxolinic acid in the topA mutant and the reaction was resistant to nalidixic acid in a topA mutant carrying, in addition, the nalA26 mutation. These results are interpreted as indicating that both DNA topoisomerase I and DNA gyrase are involved in the DNA relaxation induced by heat shock.

Original language	English
Pages (from-to)	451-455
Number of pages	5
Journal	MGG Molecular & General Genetics
Volume	244
Issue number	5
DOIs	https://doi.org/10.1007/BF00583895
Publication status	Published - 1994 Sept 1

Keywords

DNA gyrase
DNA relaxation
DNA topoisomerase I
Heat shock
topA

ASJC Scopus subject areas

Genetics

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10.1007/BF00583895

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title = "Identification of DNA topoisomerases involved in immediate and transient DNA relaxation induced by heat shock in Escherichia coli",

abstract = "The linking number of plasmid DNA in exponentially growing Escherichia coli increases immediately and transiently after heat shock. The purpose of this study was to search for DNA topoisomerases that catalyze this relaxation of DNA. Neither introduction of a topA deletion mutation nor treatment of cells with DNA gyrase inhibitors affected the DNA relaxation induced by heat shock. Thus, DNA topoisomerase I and DNA gyrase are apparently not involved in the process. However, the reaction was inhibited by nalidixic acid or by oxolinic acid in the topA mutant and the reaction was resistant to nalidixic acid in a topA mutant carrying, in addition, the nalA26 mutation. These results are interpreted as indicating that both DNA topoisomerase I and DNA gyrase are involved in the DNA relaxation induced by heat shock.",

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AU - Ogata, Yasuyuki

AU - Mizushima, Tohru

AU - Kataoka, Kazuhiro

AU - Miki, Takeyoshi

AU - Sekimizu, Kazuhisa

PY - 1994/9/1

Y1 - 1994/9/1

N2 - The linking number of plasmid DNA in exponentially growing Escherichia coli increases immediately and transiently after heat shock. The purpose of this study was to search for DNA topoisomerases that catalyze this relaxation of DNA. Neither introduction of a topA deletion mutation nor treatment of cells with DNA gyrase inhibitors affected the DNA relaxation induced by heat shock. Thus, DNA topoisomerase I and DNA gyrase are apparently not involved in the process. However, the reaction was inhibited by nalidixic acid or by oxolinic acid in the topA mutant and the reaction was resistant to nalidixic acid in a topA mutant carrying, in addition, the nalA26 mutation. These results are interpreted as indicating that both DNA topoisomerase I and DNA gyrase are involved in the DNA relaxation induced by heat shock.

AB - The linking number of plasmid DNA in exponentially growing Escherichia coli increases immediately and transiently after heat shock. The purpose of this study was to search for DNA topoisomerases that catalyze this relaxation of DNA. Neither introduction of a topA deletion mutation nor treatment of cells with DNA gyrase inhibitors affected the DNA relaxation induced by heat shock. Thus, DNA topoisomerase I and DNA gyrase are apparently not involved in the process. However, the reaction was inhibited by nalidixic acid or by oxolinic acid in the topA mutant and the reaction was resistant to nalidixic acid in a topA mutant carrying, in addition, the nalA26 mutation. These results are interpreted as indicating that both DNA topoisomerase I and DNA gyrase are involved in the DNA relaxation induced by heat shock.

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KW - DNA relaxation

KW - DNA topoisomerase I

KW - Heat shock

KW - topA

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Identification of DNA topoisomerases involved in immediate and transient DNA relaxation induced by heat shock in Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

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