Identification of genes required for neural-specific glycosylation using functional genomics.

Miki Yamamoto-Hino, Yoshimi Kanie, Wakae Awano, Kiyoko F. Aoki-Kinoshita, Hiroyuki Yano, Shoko Nishihara, Hideyuki Okano, Ryu Ueda, Osamu Kanie, Satoshi Goto

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Glycosylation plays crucial regulatory roles in various biological processes such as development, immunity, and neural functions. For example, α1,3-fucosylation, the addition of a fucose moiety abundant in Drosophila neural cells, is essential for neural development, function, and behavior. However, it remains largely unknown how neural-specific α1,3-fucosylation is regulated. In the present study, we searched for genes involved in the glycosylation of a neural-specific protein using a Drosophila RNAi library. We obtained 109 genes affecting glycosylation that clustered into nine functional groups. Among them, members of the RNA regulation group were enriched by a secondary screen that identified genes specifically regulating α1,3-fucosylation. Further analyses revealed that an RNA-binding protein, second mitotic wave missing (Swm), upregulates expression of the neural-specific glycosyltransferase FucTA and facilitates its mRNA export from the nucleus. This first large-scale genetic screen for glycosylation-related genes has revealed novel regulation of fucTA mRNA in neural cells.

Original languageEnglish
JournalPLoS Genetics
Volume6
Issue number12
DOIs
Publication statusPublished - 2010

Fingerprint

glycosylation
Genomics
Glycosylation
genomics
gene
Genes
RNA
Drosophila
genes
neurons
Biological Phenomena
Glycosyltransferases
glycosyltransferases
Messenger RNA
RNA-binding proteins
protein
RNA-Binding Proteins
Fucose
neurodevelopment
fucose

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Yamamoto-Hino, M., Kanie, Y., Awano, W., Aoki-Kinoshita, K. F., Yano, H., Nishihara, S., ... Goto, S. (2010). Identification of genes required for neural-specific glycosylation using functional genomics. PLoS Genetics, 6(12). https://doi.org/10.1371/journal.pgen.1001254

Identification of genes required for neural-specific glycosylation using functional genomics. / Yamamoto-Hino, Miki; Kanie, Yoshimi; Awano, Wakae; Aoki-Kinoshita, Kiyoko F.; Yano, Hiroyuki; Nishihara, Shoko; Okano, Hideyuki; Ueda, Ryu; Kanie, Osamu; Goto, Satoshi.

In: PLoS Genetics, Vol. 6, No. 12, 2010.

Research output: Contribution to journalArticle

Yamamoto-Hino, M, Kanie, Y, Awano, W, Aoki-Kinoshita, KF, Yano, H, Nishihara, S, Okano, H, Ueda, R, Kanie, O & Goto, S 2010, 'Identification of genes required for neural-specific glycosylation using functional genomics.', PLoS Genetics, vol. 6, no. 12. https://doi.org/10.1371/journal.pgen.1001254
Yamamoto-Hino, Miki ; Kanie, Yoshimi ; Awano, Wakae ; Aoki-Kinoshita, Kiyoko F. ; Yano, Hiroyuki ; Nishihara, Shoko ; Okano, Hideyuki ; Ueda, Ryu ; Kanie, Osamu ; Goto, Satoshi. / Identification of genes required for neural-specific glycosylation using functional genomics. In: PLoS Genetics. 2010 ; Vol. 6, No. 12.
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