Identification of genes required for neural-specific glycosylation using functional genomics

Miki Yamamoto-Hino, Yoshimi Kanie, Wakae Awano, Kiyoko F. Aoki-Kinoshita, Hiroyuki Yano, Shoko Nishihara, Hideyuki Okano, Ryu Ueda, Osamu Kanie, Satoshi Goto

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Glycosylation plays crucial regulatory roles in various biological processes such as development, immunity, and neural functions. For example, a1,3-fucosylation, the addition of a fucose moiety abundant in Drosophila neural cells, is essential for neural development, function, and behavior. However, it remains largely unknown how neural-specific a1,3-fucosylation is regulated. In the present study, we searched for genes involved in the glycosylation of a neural-specific protein using a Drosophila RNAi library. We obtained 109 genes affecting glycosylation that clustered into nine functional groups. Among them, members of the RNA regulation group were enriched by a secondary screen that identified genes specifically regulating a1,3-fucosylation. Further analyses revealed that an RNA-binding protein, second mitotic wave missing (Swm), upregulates expression of the neural-specific glycosyltransferase FucTA and facilitates its mRNA export from the nucleus. This first large-scale genetic screen for glycosylation-related genes has revealed novel regulation of fucTA mRNA in neural cells.

Original languageEnglish
Article numbere1001254
Pages (from-to)1-11
Number of pages11
JournalPLoS Genetics
Volume6
Issue number12
DOIs
Publication statusPublished - 2010 Dec

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

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    Yamamoto-Hino, M., Kanie, Y., Awano, W., Aoki-Kinoshita, K. F., Yano, H., Nishihara, S., Okano, H., Ueda, R., Kanie, O., & Goto, S. (2010). Identification of genes required for neural-specific glycosylation using functional genomics. PLoS Genetics, 6(12), 1-11. [e1001254]. https://doi.org/10.1371/journal.pgen.1001254