Identification of human cancer antigens by SEREX

Tsutomu Okada, Yutaka Kawakami

Research output: Contribution to journalArticle

Abstract

SEREX (serological identification of tumor antigens by recombinant cDNA expression cloning) is a powerful cDNA expression cloning technique for isolation of tumor antigens recognized by T cells using serum antibodies from cancer patients from whom it is difficult to raise tumor-reactive T cells. To efficiently identify tumor antigens, various modifications have been attempted for SEREX because of frequent isolation of antigens not associated with immune responses to tumor cells. These include use of a cDNA library made from testis, use of sera from patients who are immunized with autologous tumor-derived products who demonstrate tumor regression after treatment, and use of human IgG containing mice sera from immunodeficient mice implanted with human tumor tissues.

Original languageEnglish
Pages (from-to)205-211
Number of pages7
JournalBiotherapy
Volume18
Issue number3
Publication statusPublished - 2004 May 1

Keywords

  • CDNA expression cloning
  • Cancer-testis antigens
  • SEREX
  • T cells
  • Tumor antigens

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Okada, T., & Kawakami, Y. (2004). Identification of human cancer antigens by SEREX. Biotherapy, 18(3), 205-211.