Identification of human tumor antigens and its implications for diagnosis and treatment of cancer

Yutaka Kawakami, Tomonobu Fujita, Yuriko Matsuzaki, Toshiharu Sakurai, Makoto Tsukamoto, Masahiro Toda, Hidetoshi Sumimoto

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Human tumor antigens recognized by T cells have been identified by means of various molecular biological and immunological methods, including cDNA expression cloning with patients' T cells and antibodies, cDNA subtraction using RDA and PCR differential display, systematic gene analysis such as DNA sequencing, CGH, DNA chip/microarray and SAGE, in vitro T cell induction and immunization of HLA transgenic mice. The identification of human tumor antigens has led to a better understanding of the nature of tumor antigens, anti-tumor immune responses in patients before and after immunotherapy, and tumor escape mechanisms. The information obtained from these researches has enabled us to develop and improve immunotherapy by attempting to overcome the identified problems, including intrinsically low immunogenicity of tumor antigens and several escape mechanisms, such as regulatory T cell induction. The existence of immunogenic unique antigens derived from genetic alterations in tumor cells, and the varied immunogenicity of shared tumor antigens among patients due to differing expression in tumor cells and immunoreactivity of patients, indicates that individualized immunotherapy should ideally be performed. The identified antigens will also be useful for development of diagnostic methods and molecular targeting therapy for cancer.

Original languageEnglish
Pages (from-to)784-791
Number of pages8
JournalCancer Science
Volume95
Issue number10
DOIs
Publication statusPublished - 2004 Oct

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Forensic Anthropology
Neoplasm Antigens
Immunotherapy
Neoplasms
Oligonucleotide Array Sequence Analysis
T-Lymphocytes
Complementary DNA
Tumor Escape
Therapeutics
Antigens
Molecular Pathology
Regulatory T-Lymphocytes
DNA Sequence Analysis
Transgenic Mice
Organism Cloning
Immunization
Polymerase Chain Reaction
Antibodies
Research
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Identification of human tumor antigens and its implications for diagnosis and treatment of cancer. / Kawakami, Yutaka; Fujita, Tomonobu; Matsuzaki, Yuriko; Sakurai, Toshiharu; Tsukamoto, Makoto; Toda, Masahiro; Sumimoto, Hidetoshi.

In: Cancer Science, Vol. 95, No. 10, 10.2004, p. 784-791.

Research output: Contribution to journalArticle

Kawakami, Yutaka ; Fujita, Tomonobu ; Matsuzaki, Yuriko ; Sakurai, Toshiharu ; Tsukamoto, Makoto ; Toda, Masahiro ; Sumimoto, Hidetoshi. / Identification of human tumor antigens and its implications for diagnosis and treatment of cancer. In: Cancer Science. 2004 ; Vol. 95, No. 10. pp. 784-791.
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