Identification of mecA gene in MRSA by multiplex PCR

S. Hara, E. Hayashi, H. Imazeki, H. Ohtani, A. Nakata, T. Muramatsu, S. Higuchi

Research output: Contribution to journalArticle

Abstract

Methicillin-Resistant Strains of Staphylococci (MRSA) remains an important pathogen of hospital infection and the number of affected patients is rising. To limit the transmission of these organisms, early detection is crucial and Polymerase Chain Reaction (PCR) is considered to be a powerful tool for this purpose. A primary concern with PCR-based studies is whether amplified DNA may be derived from laboratory contaminants. On the contrary, negative results may be attributed to amplification errors. To overcome these problems, we developed a multiplex PCR assay using oligonucleotide primers to detect mecA and 16S ribosomal RNA gene. The results are accordant with conventional disk method or MIC method, suggesting multiplex PCR to be a useful measure for identification of MRSA.

Original languageEnglish
Pages (from-to)470-474
Number of pages5
JournalIRYO - Japanese Journal of National Medical Services
Volume52
Issue number8
Publication statusPublished - 1998 Jan 1
Externally publishedYes

Keywords

  • 16SrRNA, mecA
  • MRSA
  • PCR

ASJC Scopus subject areas

  • Medicine(all)

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    Hara, S., Hayashi, E., Imazeki, H., Ohtani, H., Nakata, A., Muramatsu, T., & Higuchi, S. (1998). Identification of mecA gene in MRSA by multiplex PCR. IRYO - Japanese Journal of National Medical Services, 52(8), 470-474.