Identification of novel Legionella genes required for endosymbiosis in Paramecium based on comparative genome analysis with Holospora spp

Kenta Watanabe, Haruo Suzuki, Takashi Nishida, Manami Mishima, Masato Tachibana, Masahiro Fujishima, Takashi Shimizu, Masahisa Watarai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The relationship between Legionella and protist hosts has a huge impact when considering the infectious risk in humans because it facilitates the long-term replication and survival of Legionella in the environment. The ciliate Paramecium is considered to be a protist host for Legionella in natural environments, but the details of their endosymbiosis are largely unknown. In this study, we determined candidate Legionella pneumophila genes that are likely to be involved in the establishment of endosymbiosis in Paramecium caudatum by comparing the genomes of Legionella spp. and Holospora spp. that are obligate endosymbiotic bacteria in Paramecium spp. Among the candidate genes, each single deletion mutant for five genes (lpg0492, lpg0522, lpg0523, lpg2141 and lpg2398) failed to establish endosymbiosis in P. caudatum despite showing intracellular growth in human macrophages. The mutants exhibited no characteristic changes in terms of their morphology, multiplication rate or capacity for modulating the phagosomes in which they were contained, but their resistance to lysozyme decreased significantly. This study provides insights into novel factors required by L. pneumophila for endosymbiosis in P. caudatum, and suggests that endosymbiotic organisms within conspecific hosts may have shared genes related to effective endosymbiosis establishment.

Original languageEnglish
JournalFEMS microbiology ecology
Volume94
Issue number11
DOIs
Publication statusPublished - 2018 Nov 1

Fingerprint

Holosporaceae
Paramecium
Legionella
Symbiosis
Paramecium caudatum
genome
Genome
gene
protist
Legionella pneumophila
Genes
ciliate
Phagosomes
Muramidase
bacterium
Macrophages
analysis
Bacteria
Survival
Growth

ASJC Scopus subject areas

  • Microbiology
  • Ecology
  • Applied Microbiology and Biotechnology

Cite this

Identification of novel Legionella genes required for endosymbiosis in Paramecium based on comparative genome analysis with Holospora spp. / Watanabe, Kenta; Suzuki, Haruo; Nishida, Takashi; Mishima, Manami; Tachibana, Masato; Fujishima, Masahiro; Shimizu, Takashi; Watarai, Masahisa.

In: FEMS microbiology ecology, Vol. 94, No. 11, 01.11.2018.

Research output: Contribution to journalArticle

Watanabe, Kenta ; Suzuki, Haruo ; Nishida, Takashi ; Mishima, Manami ; Tachibana, Masato ; Fujishima, Masahiro ; Shimizu, Takashi ; Watarai, Masahisa. / Identification of novel Legionella genes required for endosymbiosis in Paramecium based on comparative genome analysis with Holospora spp. In: FEMS microbiology ecology. 2018 ; Vol. 94, No. 11.
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