Identification of oscillatory firing neurons associated with locomotion in the earthworm through synapse imaging

T. Shimoi, K. Mizutani, D. Kojima, Y. Kitamura, Kohji Hotta, H. Ogawa, Kotaro Oka

Research output: Contribution to journalArticle

Abstract

We used FM imaging to identify neurons that receive sensory feedback from the body wall in a circuit for octopamine (OA)-evoked rhythmic locomotion in the earthworm, Eisenia fetida. We visualized synapses in which postsynaptic neurons receive the sensory feedback, by using FM1-43 dye to label the synapses of both motor and sensory pathways that are associated with locomotion, then clearing the motor pathway synapse labeling, and finally identifying the target synapses by distinguishing physiologically functional synapses through destaining using a high-K+ solution. A pair of synaptic regions associated with the sensory feedback was found to be located two or three cell body-widths away from the midline, between the anterior parts of the roots of the second lateral nerves (LNs) at the segmental ganglia (SGs). Using conventional intracellular recording and dye loading of the cell bodies surrounding these synaptic regions, we identified a pair of bilateral neurons with cell bodies larger than those of other cells in these regions, and named them "Oscillatory firing neurons Projecting to Peripheral nerves" (OPPs). These had a bipolar shape and projected neurites to the ipsilateral first and third LNs, fired rhythmically, and had a burst timing synchronized with the motor pattern bursts from the ipsilateral first LNs. Current injection into an OPP caused firing in the ipsilateral first LNs, supporting the hypothesis that OPPs functionally project to the peripheral nerves. OPPs also sent neurites to the adjacent anterior and posterior SGs, suggesting connections with the adjacent segments. We conclude that FM imaging can be used to identify neurons involved in specific functions, and that OPPs are the first neurons to be associated with OA-induced locomotion in the earthworm.

Original languageEnglish
Pages (from-to)149-158
Number of pages10
JournalNeuroscience
Volume268
DOIs
Publication statusPublished - 2014 May 30

Fingerprint

Oligochaeta
Locomotion
Synapses
Neurons
Sensory Feedback
Octopamine
Efferent Pathways
Neurites
Peripheral Nerves
Ganglia
Coloring Agents
4-(2-oxapentadeca-4-yne)phenylpropanoic acid
Injections
Cell Body

Keywords

  • Activity-dependent staining
  • FM1-43
  • Octopamine
  • Rhythmic behavior
  • Screening for functional neurons
  • Sensory feedback

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Identification of oscillatory firing neurons associated with locomotion in the earthworm through synapse imaging. / Shimoi, T.; Mizutani, K.; Kojima, D.; Kitamura, Y.; Hotta, Kohji; Ogawa, H.; Oka, Kotaro.

In: Neuroscience, Vol. 268, 30.05.2014, p. 149-158.

Research output: Contribution to journalArticle

Shimoi, T. ; Mizutani, K. ; Kojima, D. ; Kitamura, Y. ; Hotta, Kohji ; Ogawa, H. ; Oka, Kotaro. / Identification of oscillatory firing neurons associated with locomotion in the earthworm through synapse imaging. In: Neuroscience. 2014 ; Vol. 268. pp. 149-158.
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