Identification of poly(rC) binding protein 2 (PCBP2) as a target protein of immunosuppressive agent 15-deoxyspergualin

Masataka Murahashi, Siro Simizu, Masahiko Morioka, Kazuo Umezawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

15-Deoxyspergualin (DSG) is an immunosuppressive agent being clinically used. Unlike tacrolimus and cyclosporine A, it does not inhibit the calcineurin pathway, and its mechanism of action and target molecule have not been elucidated. Therefore, we previously prepared biotinylated derivative of DSG (BDSG) to fish up the target protein. In the present research, we identified poly(rC) binding protein 2 (PCBP2) as a DSG-binding protein using this probe. DSG was confirmed to bind to PCBP2 by pull-down assay. Intracellular localization of PCBP2 was changed from the nucleus to the cytoplasm by DSG treatment. DSG inhibited the cell growth, and over-expression of PCBP2 reduced the anti-proliferative activity of DSG. PCBP2 is known to regulate various proteins including STAT1/2. Thus, we found PCBP2 as the first target protein of DSG that can explain the immunosuppressive activity.

Original languageEnglish
Pages (from-to)445-449
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume476
Issue number4
DOIs
Publication statusPublished - 2016 Aug 5

Keywords

  • 15-Deoxyspergualin
  • Biotin conjugate
  • Poly(rC) binding protein 2 (PCBP2)
  • Signal transducer and activator of transcription (STAT)
  • Target protein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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