Identification of proteins whose amounts are altered by mutation in the pgsA gene of Escherichia coli

Akiko Ohba, Ryu Ichi Inoue, Nobuyoshi Akimitsu, Tohru Mizushima, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We identified proteins whose amounts were altered in an Escherichia coli pgsA3 mutant lacking the potential to synthesize phosphatidylglycerolphosphate, a precursor of phosphatidylglycerol. Proteins whose amounts were increased in the mutant were protease Do, periplasmic oligopeptide-binding protein, tryptophanase, and an unidentified protein, while the decreased one was flagellin. Transformation of the mutant with a plasmid containing the wild type pgsA gene complemented the phenotype, indicating that the pgsA3 mutation is responsible for the phenotype.

Original languageEnglish
Pages (from-to)1139-1141
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume21
Issue number11
Publication statusPublished - 1998 Nov
Externally publishedYes

Fingerprint

Escherichia coli
Mutation
Tryptophanase
Periplasmic Binding Proteins
Genes
Phenotype
Flagellin
Oligopeptides
Phosphatidylglycerols
Proteins
Plasmids
DegP protease

Keywords

  • Escherichia coli
  • Phosphatidylglycerol
  • Two-dimensional polyacrylamide gel electrophoresis

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Ohba, A., Inoue, R. I., Akimitsu, N., Mizushima, T., & Sekimizu, K. (1998). Identification of proteins whose amounts are altered by mutation in the pgsA gene of Escherichia coli. Biological and Pharmaceutical Bulletin, 21(11), 1139-1141.

Identification of proteins whose amounts are altered by mutation in the pgsA gene of Escherichia coli. / Ohba, Akiko; Inoue, Ryu Ichi; Akimitsu, Nobuyoshi; Mizushima, Tohru; Sekimizu, Kazuhisa.

In: Biological and Pharmaceutical Bulletin, Vol. 21, No. 11, 11.1998, p. 1139-1141.

Research output: Contribution to journalArticle

Ohba, A, Inoue, RI, Akimitsu, N, Mizushima, T & Sekimizu, K 1998, 'Identification of proteins whose amounts are altered by mutation in the pgsA gene of Escherichia coli', Biological and Pharmaceutical Bulletin, vol. 21, no. 11, pp. 1139-1141.
Ohba, Akiko ; Inoue, Ryu Ichi ; Akimitsu, Nobuyoshi ; Mizushima, Tohru ; Sekimizu, Kazuhisa. / Identification of proteins whose amounts are altered by mutation in the pgsA gene of Escherichia coli. In: Biological and Pharmaceutical Bulletin. 1998 ; Vol. 21, No. 11. pp. 1139-1141.
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