Identification of proteins whose amounts are altered by mutation in the pgsA gene of Escherichia coli

Akiko Ohba, Ryu Ichi Inoue, Nobuyoshi Akimitsu, Tohru Mizushima, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We identified proteins whose amounts were altered in an Escherichia coli pgsA3 mutant lacking the potential to synthesize phosphatidylglycerolphosphate, a precursor of phosphatidylglycerol. Proteins whose amounts were increased in the mutant were protease Do, periplasmic oligopeptide-binding protein, tryptophanase, and an unidentified protein, while the decreased one was flagellin. Transformation of the mutant with a plasmid containing the wild type pgsA gene complemented the phenotype, indicating that the pgsA3 mutation is responsible for the phenotype.

Original languageEnglish
Pages (from-to)1139-1141
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume21
Issue number11
DOIs
Publication statusPublished - 1998 Nov

Keywords

  • Escherichia coli
  • Phosphatidylglycerol
  • Two-dimensional polyacrylamide gel electrophoresis

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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