Identification of Semaphorin3B as a direct target of p53

Kensuke Ochi, Toshiki Mori, Yoshiaki Toyama, Yusuke Nakamura, Hirofumi Arakawa

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Abstract

A cDNA microarray analysis indicated that Semaphorin3B (Sema3B), a gene whose product is involved in axon guidance and axonal repulsion, is inducible by p53. Introduction of exogenous p53 into a glioblastoma cell line lacking wild-type p53 (U373MG) dramatically induced expression of Sema3B mRNA. An electrophoretic mobility shift assay and a reporter assay confirmed that a potential p53 binding site present in the promoter region had p53-dependent transcriptional activity. Expression of endogenous Sema3B was induced in response to genotoxic stresses caused by adriamycin treatment or UV irradiation in a p53-dependent manner. Ectopic expression of Sema3B in p53-defective cells reduced the number of colonies in colony formation assays. These results suggest that Sema3B might play some role in regulating cell growth as a mediator of p53 tumor-suppressor activity.

Original languageEnglish
Pages (from-to)82-87
Number of pages6
JournalNeoplasia
Volume4
Issue number1
DOIs
Publication statusPublished - 2002 Jan 29

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Keywords

  • Apoptosis
  • Growth suppression
  • Semaphorin3B
  • Target gene
  • p53

ASJC Scopus subject areas

  • Cancer Research

Cite this

Ochi, K., Mori, T., Toyama, Y., Nakamura, Y., & Arakawa, H. (2002). Identification of Semaphorin3B as a direct target of p53. Neoplasia, 4(1), 82-87. https://doi.org/10.1038/sj/neo/7900211