Identification of Semaphorin3B as a direct target of p53

Kensuke Ochi, Toshiki Mori, Yoshiaki Toyama, Yusuke Nakamura, Hirofumi Arakawa

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

A cDNA microarray analysis indicated that Semaphorin3B (Sema3B), a gene whose product is involved in axon guidance and axonal repulsion, is inducible by p53. Introduction of exogenous p53 into a glioblastoma cell line lacking wild-type p53 (U373MG) dramatically induced expression of Sema3B mRNA. An electrophoretic mobility shift assay and a reporter assay confirmed that a potential p53 binding site present in the promoter region had p53-dependent transcriptional activity. Expression of endogenous Sema3B was induced in response to genotoxic stresses caused by adriamycin treatment or UV irradiation in a p53-dependent manner. Ectopic expression of Sema3B in p53-defective cells reduced the number of colonies in colony formation assays. These results suggest that Sema3B might play some role in regulating cell growth as a mediator of p53 tumor-suppressor activity.

Original languageEnglish
Pages (from-to)82-87
Number of pages6
JournalNeoplasia
Volume4
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

Electrophoretic Mobility Shift Assay
Glioblastoma
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Genetic Promoter Regions
Doxorubicin
DNA Damage
Cell Count
Binding Sites
Cell Line
Messenger RNA
Growth
Genes
Neoplasms
Axon Guidance
Ectopic Gene Expression

Keywords

  • Apoptosis
  • Growth suppression
  • p53
  • Semaphorin3B
  • Target gene

ASJC Scopus subject areas

  • Cancer Research

Cite this

Ochi, K., Mori, T., Toyama, Y., Nakamura, Y., & Arakawa, H. (2002). Identification of Semaphorin3B as a direct target of p53. Neoplasia, 4(1), 82-87. https://doi.org/10.1038/sj/neo/7900211

Identification of Semaphorin3B as a direct target of p53. / Ochi, Kensuke; Mori, Toshiki; Toyama, Yoshiaki; Nakamura, Yusuke; Arakawa, Hirofumi.

In: Neoplasia, Vol. 4, No. 1, 2002, p. 82-87.

Research output: Contribution to journalArticle

Ochi, K, Mori, T, Toyama, Y, Nakamura, Y & Arakawa, H 2002, 'Identification of Semaphorin3B as a direct target of p53', Neoplasia, vol. 4, no. 1, pp. 82-87. https://doi.org/10.1038/sj/neo/7900211
Ochi K, Mori T, Toyama Y, Nakamura Y, Arakawa H. Identification of Semaphorin3B as a direct target of p53. Neoplasia. 2002;4(1):82-87. https://doi.org/10.1038/sj/neo/7900211
Ochi, Kensuke ; Mori, Toshiki ; Toyama, Yoshiaki ; Nakamura, Yusuke ; Arakawa, Hirofumi. / Identification of Semaphorin3B as a direct target of p53. In: Neoplasia. 2002 ; Vol. 4, No. 1. pp. 82-87.
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