Identification of the cis-acting region in the NF2 gene promoter as a potential target for mutation and methylation-dependent silencing in schwannoma

Takeshi Kino, Hideo Takeshima, Mitsuyoshi Nakao, Toru Nishi, Keizo Yamamoto, Takahiro Kimura, Yoshiki Saito, Masato Kochi, Jun Ichi Kuratsu, Hideyuki Saya, Yukitaka Ushio

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Abstract

Background: Although mutational inactivation and allelic loss in the NF2 gene appear to be causal events in the majority of vestibular schwannomas, involvement of another potentially important mechanism, transcriptional inactivation, has not been investigated. Results: We cloned and functionally characterized the 5′-flanking region of the human NF2 gene and identified the molecular mechanisms that regulate NF2 expression. Luciferase assay and site-directed mutagenesis demonstrated that a 70-base pair (bp) region (-591 to -522 bp from the translation start site) was essential for the basic expression of the NF2 gene. A gel mobility shift assay indicated recognition by nuclear protein of the unusually long (≈66 bp) sequences in this region. Recognition was inhibited by either mutation of the binding core sequence or by methylation of three CpG sites. Point mutations at these CpG sites significantly decreased promoter activity, suggesting the importance of these sites. In 14 of 23 vestibular schwannomas, these three CpG sites were methylated in a site-specific manner and the methylation status was consistent with the expression of NF2 mRNA. Conclusions: Suppressed expression by aberrant methylation or mutation of the promoter elements could be an alternative mechanism for inactivation of the NF2 gene.

Original languageEnglish
Pages (from-to)441-454
Number of pages14
JournalGenes to Cells
Volume6
Issue number5
DOIs
Publication statusPublished - 2001 Jun 9
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Kino, T., Takeshima, H., Nakao, M., Nishi, T., Yamamoto, K., Kimura, T., Saito, Y., Kochi, M., Kuratsu, J. I., Saya, H., & Ushio, Y. (2001). Identification of the cis-acting region in the NF2 gene promoter as a potential target for mutation and methylation-dependent silencing in schwannoma. Genes to Cells, 6(5), 441-454. https://doi.org/10.1046/j.1365-2443.2001.00432.x