Identification of the immunodominant peptides of the MART-1 human melanoma antigen recognized by the majority of HLA-A2-restricted tumor infiltrating lymphocytes

Yutaka Kawakami, Siona Eliyahu, Kazuyasu Sakaguchi, Paul F. Robbins, Licia Rivoltini, John R. Yannelli, Ettore Appella, Steven A. Rosenberg

Research output: Contribution to journalArticle

722 Citations (Scopus)

Abstract

Four melanoma proteins, MART-1, gp100, tyrosinase, and tyrosinase-related protein-1 (gp75) were evaluated for recognition by HLA-A2-restricted melanoma-specific cytotoxic T lymphocytes (CTLs) derived from the tumor- infiltrating lymphocytes (TIL) of 10 different patients. 9 of 10 TIL recognized MART-1, 4 recognized gp100 (including 3 that also recognized MART- 1), but none of the TIL recognized tyrosinase or gp75. Based on the known HLA-A2.1 peptide binding motifs, 23 peptides from MART-1 were synthesized in an attempt to identify the epitopes recognized by TIL. Three peptides were recognized by TIL when pulsed on T2 target cells. One of the 9-mer peptides, AAGIGILTV, was most effective in sensitizing the T2 cells for TIL lysis. This peptide was recognized by 9 of 10 HLA-A2-restricted melanoma-specific CTLs. Therefore, this peptide appears to be a very common immunogenic epitope for HLA-A2-restricted melanoma-specific TIL and may be useful for the development of immunotherapeutic strategies.

Original languageEnglish
Pages (from-to)347-352
Number of pages6
JournalJournal of Experimental Medicine
Volume180
Issue number1
DOIs
Publication statusPublished - 1994 Jul 1
Externally publishedYes

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Tumor-Infiltrating Lymphocytes
HLA-A2 Antigen
Peptides
Melanoma
Monophenol Monooxygenase
Cytotoxic T-Lymphocytes
Epitopes
human MAGEA1 protein

ASJC Scopus subject areas

  • Immunology

Cite this

Identification of the immunodominant peptides of the MART-1 human melanoma antigen recognized by the majority of HLA-A2-restricted tumor infiltrating lymphocytes. / Kawakami, Yutaka; Eliyahu, Siona; Sakaguchi, Kazuyasu; Robbins, Paul F.; Rivoltini, Licia; Yannelli, John R.; Appella, Ettore; Rosenberg, Steven A.

In: Journal of Experimental Medicine, Vol. 180, No. 1, 01.07.1994, p. 347-352.

Research output: Contribution to journalArticle

Kawakami, Yutaka ; Eliyahu, Siona ; Sakaguchi, Kazuyasu ; Robbins, Paul F. ; Rivoltini, Licia ; Yannelli, John R. ; Appella, Ettore ; Rosenberg, Steven A. / Identification of the immunodominant peptides of the MART-1 human melanoma antigen recognized by the majority of HLA-A2-restricted tumor infiltrating lymphocytes. In: Journal of Experimental Medicine. 1994 ; Vol. 180, No. 1. pp. 347-352.
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