Abstract
A 2.4-kilobase fragment of the Bacillus subtilis chromosome containing the wild-type spoOA gene derived from the φ105dspoOA+-Bc-1 transducing phage was cloned onto plasmid pBR322 in Escherichia coli. A recombinant plasmid harboring the mutant spoOA12 allele on the 2.4-kilobase insert was also constructed from the φ105dspoOA12-1 phage DNA and pBR322. Protein products synthesized in reponse to plasmid DNA in a DNA-directed cell-free system derived from E. coli were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A protein of approximately 27,500 daltons synthesized with the recombinant plasmid DNA harboring the wild-type spoOA gene as template was not formed with the recombinant plasmid DNA harboring the spoOA12 allele. Since the spoOA12 mutation is a nonsense mutation, we conclude that the 27.5-kilodalton protein is the product of the spoOA gene.
| Original language | English |
|---|---|
| Pages (from-to) | 1104-1109 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 122 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1984 Aug 16 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Identification of the sporulation gene spoOA product of Bacillus subtilis. / Kudo, Jun; Ikeuchi, Toshihiko; Kurahashi, Kiyoshi.
In: Biochemical and Biophysical Research Communications, Vol. 122, No. 3, 16.08.1984, p. 1104-1109.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of the sporulation gene spoOA product of Bacillus subtilis
AU - Kudo, Jun
AU - Ikeuchi, Toshihiko
AU - Kurahashi, Kiyoshi
PY - 1984/8/16
Y1 - 1984/8/16
N2 - A 2.4-kilobase fragment of the Bacillus subtilis chromosome containing the wild-type spoOA gene derived from the φ105dspoOA+-Bc-1 transducing phage was cloned onto plasmid pBR322 in Escherichia coli. A recombinant plasmid harboring the mutant spoOA12 allele on the 2.4-kilobase insert was also constructed from the φ105dspoOA12-1 phage DNA and pBR322. Protein products synthesized in reponse to plasmid DNA in a DNA-directed cell-free system derived from E. coli were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A protein of approximately 27,500 daltons synthesized with the recombinant plasmid DNA harboring the wild-type spoOA gene as template was not formed with the recombinant plasmid DNA harboring the spoOA12 allele. Since the spoOA12 mutation is a nonsense mutation, we conclude that the 27.5-kilodalton protein is the product of the spoOA gene.
AB - A 2.4-kilobase fragment of the Bacillus subtilis chromosome containing the wild-type spoOA gene derived from the φ105dspoOA+-Bc-1 transducing phage was cloned onto plasmid pBR322 in Escherichia coli. A recombinant plasmid harboring the mutant spoOA12 allele on the 2.4-kilobase insert was also constructed from the φ105dspoOA12-1 phage DNA and pBR322. Protein products synthesized in reponse to plasmid DNA in a DNA-directed cell-free system derived from E. coli were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A protein of approximately 27,500 daltons synthesized with the recombinant plasmid DNA harboring the wild-type spoOA gene as template was not formed with the recombinant plasmid DNA harboring the spoOA12 allele. Since the spoOA12 mutation is a nonsense mutation, we conclude that the 27.5-kilodalton protein is the product of the spoOA gene.
UR - http://www.scopus.com/inward/record.url?scp=0021765489&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021765489&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(84)91205-1
DO - 10.1016/0006-291X(84)91205-1
M3 - Article
VL - 122
SP - 1104
EP - 1109
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -