Identification of the sporulation gene spoOA product of Bacillus subtilis

Jun Kudoh, Toshihiko Ikeuchi, Kiyoshi Kurahashi

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

A 2.4-kilobase fragment of the Bacillus subtilis chromosome containing the wild-type spoOA gene derived from the φ105dspoOA+-Bc-1 transducing phage was cloned onto plasmid pBR322 in Escherichia coli. A recombinant plasmid harboring the mutant spoOA12 allele on the 2.4-kilobase insert was also constructed from the φ105dspoOA12-1 phage DNA and pBR322. Protein products synthesized in reponse to plasmid DNA in a DNA-directed cell-free system derived from E. coli were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A protein of approximately 27,500 daltons synthesized with the recombinant plasmid DNA harboring the wild-type spoOA gene as template was not formed with the recombinant plasmid DNA harboring the spoOA12 allele. Since the spoOA12 mutation is a nonsense mutation, we conclude that the 27.5-kilodalton protein is the product of the spoOA gene.

Original languageEnglish
Pages (from-to)1104-1109
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume122
Issue number3
DOIs
Publication statusPublished - 1984 Aug 16

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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