Identification of the sporulation gene spoOA product of Bacillus subtilis

Jun Kudo, Toshihiko Ikeuchi, Kiyoshi Kurahashi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A 2.4-kilobase fragment of the Bacillus subtilis chromosome containing the wild-type spoOA gene derived from the φ105dspoOA+-Bc-1 transducing phage was cloned onto plasmid pBR322 in Escherichia coli. A recombinant plasmid harboring the mutant spoOA12 allele on the 2.4-kilobase insert was also constructed from the φ105dspoOA12-1 phage DNA and pBR322. Protein products synthesized in reponse to plasmid DNA in a DNA-directed cell-free system derived from E. coli were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A protein of approximately 27,500 daltons synthesized with the recombinant plasmid DNA harboring the wild-type spoOA gene as template was not formed with the recombinant plasmid DNA harboring the spoOA12 allele. Since the spoOA12 mutation is a nonsense mutation, we conclude that the 27.5-kilodalton protein is the product of the spoOA gene.

Original languageEnglish
Pages (from-to)1104-1109
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume122
Issue number3
DOIs
Publication statusPublished - 1984 Aug 16
Externally publishedYes

Fingerprint

Bacilli
Bacillus subtilis
Plasmids
Genes
Bacteriophages
Recombinant DNA
Escherichia coli
DNA
Alleles
Proteins
Cell-Free System
Nonsense Codon
Chromosomes
Electrophoresis
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Mutation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of the sporulation gene spoOA product of Bacillus subtilis. / Kudo, Jun; Ikeuchi, Toshihiko; Kurahashi, Kiyoshi.

In: Biochemical and Biophysical Research Communications, Vol. 122, No. 3, 16.08.1984, p. 1104-1109.

Research output: Contribution to journalArticle

Kudo, Jun ; Ikeuchi, Toshihiko ; Kurahashi, Kiyoshi. / Identification of the sporulation gene spoOA product of Bacillus subtilis. In: Biochemical and Biophysical Research Communications. 1984 ; Vol. 122, No. 3. pp. 1104-1109.
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