TY - JOUR
T1 - Identification of the sporulation gene spoOA product of Bacillus subtilis
AU - Kudoh, Jun
AU - Ikeuchi, Toshihiko
AU - Kurahashi, Kiyoshi
N1 - Funding Information:
We are grateful to J. Spizizin, P. Schaeffer, Sugino, H. Ogawa, M. Yamada and the Bacillus Genetic ing us with the bacterial strains used in this study members of this laboratory for helpful discussions. This research was supported by Grants-in-Aid from the Ministry of Education, Science and Culture
PY - 1984/8/16
Y1 - 1984/8/16
N2 - A 2.4-kilobase fragment of the Bacillus subtilis chromosome containing the wild-type spoOA gene derived from the φ105dspoOA+-Bc-1 transducing phage was cloned onto plasmid pBR322 in Escherichia coli. A recombinant plasmid harboring the mutant spoOA12 allele on the 2.4-kilobase insert was also constructed from the φ105dspoOA12-1 phage DNA and pBR322. Protein products synthesized in reponse to plasmid DNA in a DNA-directed cell-free system derived from E. coli were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A protein of approximately 27,500 daltons synthesized with the recombinant plasmid DNA harboring the wild-type spoOA gene as template was not formed with the recombinant plasmid DNA harboring the spoOA12 allele. Since the spoOA12 mutation is a nonsense mutation, we conclude that the 27.5-kilodalton protein is the product of the spoOA gene.
AB - A 2.4-kilobase fragment of the Bacillus subtilis chromosome containing the wild-type spoOA gene derived from the φ105dspoOA+-Bc-1 transducing phage was cloned onto plasmid pBR322 in Escherichia coli. A recombinant plasmid harboring the mutant spoOA12 allele on the 2.4-kilobase insert was also constructed from the φ105dspoOA12-1 phage DNA and pBR322. Protein products synthesized in reponse to plasmid DNA in a DNA-directed cell-free system derived from E. coli were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A protein of approximately 27,500 daltons synthesized with the recombinant plasmid DNA harboring the wild-type spoOA gene as template was not formed with the recombinant plasmid DNA harboring the spoOA12 allele. Since the spoOA12 mutation is a nonsense mutation, we conclude that the 27.5-kilodalton protein is the product of the spoOA gene.
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U2 - 10.1016/0006-291X(84)91205-1
DO - 10.1016/0006-291X(84)91205-1
M3 - Article
C2 - 6089776
AN - SCOPUS:0021765489
SN - 0006-291X
VL - 122
SP - 1104
EP - 1109
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -