Identification of TOSO/FAIM3 as an Fc receptor for IgM

Hideaki Shima, Hiroyuki Takatsu, Shinji Fukuda, Masumi Ohmae, Kouji Hase, Hiromi Kubagawa, Ji Yang Wang, Hiroshi Ohno

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Fc receptors specifically bind to the Fc region of Igs to mediate the unique functions to each class of Igs. To identify a novel Fc receptor for IgM, we searched expressed sequence tag database for molecules containing Ig domains with homology to those of known Fc receptors for IgM, Fca/μR and polymeric Ig receptor. As a result, we identified TOSO/Fas apoptotic inhibitory molecule 3 (FAIM3) as a possible Fc receptor for IgM. HeLa cells transfected with a TOSO/FAIM3-expression vector bound to IgM but not IgG and were able to internalize IgM-conjugated beads but not IgG-conjugated beads, suggesting that TOSO/FAIM3 is indeed a receptor for IgM (FcμR). FcmR protein was expressed predominantly on B-lineage cells; expression of the Fcmr transcripts was observed from the pre-β-cell stage and maintained thereafter during B-cell development. These results identify TOSO/FAIM3 as a receptor for IgM and suggest that FcμR may serve as an uptake receptor for IgM-opsonized antigens by B cells.

Original languageEnglish
Article numberdxp121
Pages (from-to)149-156
Number of pages8
JournalInternational Immunology
Volume22
Issue number3
DOIs
Publication statusPublished - 2009 Dec 30
Externally publishedYes

Fingerprint

Immunoglobulin M
B-Lymphocytes
Polymeric Immunoglobulin Receptors
Immunoglobulin G
Fc Receptors
Expressed Sequence Tags
HeLa Cells
immunoglobulin M receptor
Databases
Antigens
Proteins

Keywords

  • B cell
  • Fc receptor
  • IgM
  • TOSO/FAIM3

ASJC Scopus subject areas

  • Immunology

Cite this

Shima, H., Takatsu, H., Fukuda, S., Ohmae, M., Hase, K., Kubagawa, H., ... Ohno, H. (2009). Identification of TOSO/FAIM3 as an Fc receptor for IgM. International Immunology, 22(3), 149-156. [dxp121]. https://doi.org/10.1093/intimm/dxp121

Identification of TOSO/FAIM3 as an Fc receptor for IgM. / Shima, Hideaki; Takatsu, Hiroyuki; Fukuda, Shinji; Ohmae, Masumi; Hase, Kouji; Kubagawa, Hiromi; Wang, Ji Yang; Ohno, Hiroshi.

In: International Immunology, Vol. 22, No. 3, dxp121, 30.12.2009, p. 149-156.

Research output: Contribution to journalArticle

Shima, H, Takatsu, H, Fukuda, S, Ohmae, M, Hase, K, Kubagawa, H, Wang, JY & Ohno, H 2009, 'Identification of TOSO/FAIM3 as an Fc receptor for IgM', International Immunology, vol. 22, no. 3, dxp121, pp. 149-156. https://doi.org/10.1093/intimm/dxp121
Shima H, Takatsu H, Fukuda S, Ohmae M, Hase K, Kubagawa H et al. Identification of TOSO/FAIM3 as an Fc receptor for IgM. International Immunology. 2009 Dec 30;22(3):149-156. dxp121. https://doi.org/10.1093/intimm/dxp121
Shima, Hideaki ; Takatsu, Hiroyuki ; Fukuda, Shinji ; Ohmae, Masumi ; Hase, Kouji ; Kubagawa, Hiromi ; Wang, Ji Yang ; Ohno, Hiroshi. / Identification of TOSO/FAIM3 as an Fc receptor for IgM. In: International Immunology. 2009 ; Vol. 22, No. 3. pp. 149-156.
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