Identification of transcription factors for lineage-specific ESC differentiation

Kohei Yamamizu, Yulan Piao, Alexei A. Sharov, Veronika Zsiros, Hong Yu, Kazu Nakazawa, David Schlessinger, Minoru Ko

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A network of transcription factors (TFs) determines cell identity, but identity can be altered by overexpressing a combination of TFs. However, choosing and verifying combinations of TFs for specific cell differentiation have been daunting due to the large number of possible combinations of ∼2,000 TFs. Here, we report the identification of individual TFs for lineage-specific cell differentiation based on the correlation matrix of global gene expression profiles. The overexpression of identified TFs - Myod1, Mef2c, Esx1, Foxa1, Hnf4a, Gata2, Gata3, Myc, Elf5, Irf2, Elf1, Sfpi1, Ets1, Smad7, Nr2f1, Sox11, Dmrt1, Sox9, Foxg1, Sox2, or Ascl1 - can direct efficient, specific, and rapid differentiation into myocytes, hepatocytes, blood cells, and neurons. Furthermore, transfection of synthetic mRNAs of TFs generates their appropriate target cells. These results demonstrate both the utility of this approach to identify potent TFs for cell differentiation, and the unanticipated capacity of single TFs directly guides differentiation to specific lineage fates.

Original languageEnglish
Pages (from-to)545-559
Number of pages15
JournalStem Cell Reports
Volume1
Issue number6
DOIs
Publication statusPublished - 2013 Dec 17

Fingerprint

Transcription Factors
Cell Differentiation
Transcriptome
Gene expression
Muscle Cells
Neurons
Transfection
Hepatocytes
Blood Cells
Blood
Cells
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Developmental Biology
  • Genetics
  • Medicine(all)

Cite this

Identification of transcription factors for lineage-specific ESC differentiation. / Yamamizu, Kohei; Piao, Yulan; Sharov, Alexei A.; Zsiros, Veronika; Yu, Hong; Nakazawa, Kazu; Schlessinger, David; Ko, Minoru.

In: Stem Cell Reports, Vol. 1, No. 6, 17.12.2013, p. 545-559.

Research output: Contribution to journalArticle

Yamamizu, K, Piao, Y, Sharov, AA, Zsiros, V, Yu, H, Nakazawa, K, Schlessinger, D & Ko, M 2013, 'Identification of transcription factors for lineage-specific ESC differentiation', Stem Cell Reports, vol. 1, no. 6, pp. 545-559. https://doi.org/10.1016/j.stemcr.2013.10.006
Yamamizu K, Piao Y, Sharov AA, Zsiros V, Yu H, Nakazawa K et al. Identification of transcription factors for lineage-specific ESC differentiation. Stem Cell Reports. 2013 Dec 17;1(6):545-559. https://doi.org/10.1016/j.stemcr.2013.10.006
Yamamizu, Kohei ; Piao, Yulan ; Sharov, Alexei A. ; Zsiros, Veronika ; Yu, Hong ; Nakazawa, Kazu ; Schlessinger, David ; Ko, Minoru. / Identification of transcription factors for lineage-specific ESC differentiation. In: Stem Cell Reports. 2013 ; Vol. 1, No. 6. pp. 545-559.
@article{12e5b236f00f4911b600e5315524d26b,
title = "Identification of transcription factors for lineage-specific ESC differentiation",
abstract = "A network of transcription factors (TFs) determines cell identity, but identity can be altered by overexpressing a combination of TFs. However, choosing and verifying combinations of TFs for specific cell differentiation have been daunting due to the large number of possible combinations of ∼2,000 TFs. Here, we report the identification of individual TFs for lineage-specific cell differentiation based on the correlation matrix of global gene expression profiles. The overexpression of identified TFs - Myod1, Mef2c, Esx1, Foxa1, Hnf4a, Gata2, Gata3, Myc, Elf5, Irf2, Elf1, Sfpi1, Ets1, Smad7, Nr2f1, Sox11, Dmrt1, Sox9, Foxg1, Sox2, or Ascl1 - can direct efficient, specific, and rapid differentiation into myocytes, hepatocytes, blood cells, and neurons. Furthermore, transfection of synthetic mRNAs of TFs generates their appropriate target cells. These results demonstrate both the utility of this approach to identify potent TFs for cell differentiation, and the unanticipated capacity of single TFs directly guides differentiation to specific lineage fates.",
author = "Kohei Yamamizu and Yulan Piao and Sharov, {Alexei A.} and Veronika Zsiros and Hong Yu and Kazu Nakazawa and David Schlessinger and Minoru Ko",
year = "2013",
month = "12",
day = "17",
doi = "10.1016/j.stemcr.2013.10.006",
language = "English",
volume = "1",
pages = "545--559",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "6",

}

TY - JOUR

T1 - Identification of transcription factors for lineage-specific ESC differentiation

AU - Yamamizu, Kohei

AU - Piao, Yulan

AU - Sharov, Alexei A.

AU - Zsiros, Veronika

AU - Yu, Hong

AU - Nakazawa, Kazu

AU - Schlessinger, David

AU - Ko, Minoru

PY - 2013/12/17

Y1 - 2013/12/17

N2 - A network of transcription factors (TFs) determines cell identity, but identity can be altered by overexpressing a combination of TFs. However, choosing and verifying combinations of TFs for specific cell differentiation have been daunting due to the large number of possible combinations of ∼2,000 TFs. Here, we report the identification of individual TFs for lineage-specific cell differentiation based on the correlation matrix of global gene expression profiles. The overexpression of identified TFs - Myod1, Mef2c, Esx1, Foxa1, Hnf4a, Gata2, Gata3, Myc, Elf5, Irf2, Elf1, Sfpi1, Ets1, Smad7, Nr2f1, Sox11, Dmrt1, Sox9, Foxg1, Sox2, or Ascl1 - can direct efficient, specific, and rapid differentiation into myocytes, hepatocytes, blood cells, and neurons. Furthermore, transfection of synthetic mRNAs of TFs generates their appropriate target cells. These results demonstrate both the utility of this approach to identify potent TFs for cell differentiation, and the unanticipated capacity of single TFs directly guides differentiation to specific lineage fates.

AB - A network of transcription factors (TFs) determines cell identity, but identity can be altered by overexpressing a combination of TFs. However, choosing and verifying combinations of TFs for specific cell differentiation have been daunting due to the large number of possible combinations of ∼2,000 TFs. Here, we report the identification of individual TFs for lineage-specific cell differentiation based on the correlation matrix of global gene expression profiles. The overexpression of identified TFs - Myod1, Mef2c, Esx1, Foxa1, Hnf4a, Gata2, Gata3, Myc, Elf5, Irf2, Elf1, Sfpi1, Ets1, Smad7, Nr2f1, Sox11, Dmrt1, Sox9, Foxg1, Sox2, or Ascl1 - can direct efficient, specific, and rapid differentiation into myocytes, hepatocytes, blood cells, and neurons. Furthermore, transfection of synthetic mRNAs of TFs generates their appropriate target cells. These results demonstrate both the utility of this approach to identify potent TFs for cell differentiation, and the unanticipated capacity of single TFs directly guides differentiation to specific lineage fates.

UR - http://www.scopus.com/inward/record.url?scp=84890899156&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84890899156&partnerID=8YFLogxK

U2 - 10.1016/j.stemcr.2013.10.006

DO - 10.1016/j.stemcr.2013.10.006

M3 - Article

VL - 1

SP - 545

EP - 559

JO - Stem Cell Reports

JF - Stem Cell Reports

SN - 2213-6711

IS - 6

ER -