Identification of Trichophyton rubrum by nested PCR analysis from paraffin embedded specimen in trichophytia profunda acuta of the glabrous skin

Keisuke Nagao, Takashi Sugita, Takeshi Ouchi, Takeji Nishikawa

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Trichophytia profunda acuta of the glabrous skin (TPAGS) arose in a 67-year-old Japanese man. The patient presented indurated erythematous plaques and nodules on his left forearm. Direct microscopic examination of the scale in KOH preparation was negative for fungal elements, and culture for dermatophytes was also negative. Although fungal infection could not be proven in hematoxillin-eosin stained sections, deep-cut sections of the biopsied skin lesion with PAS stain revealed the ectothrix presence of fungal elements. Nested PCR was done with Trichophyton specific primers directed to internal transcribed spacer gene 1 (ITS1), using template DNA obtained from formalin fixed, paraffin embedded skin sections. A single band corresponding to T. rubrum was obtained, and the etiological agent was thus identified. KOH tests and cultures may often turn out unsuccessful, perhaps reflecting the hair follicle dominant fungus growth in TPAGS. Although these tests are most important for diagnosis of TPAGS, nested PCR using paraffin embedded skin sections may be an alternative method to identify the etiological agent.

Original languageEnglish
Pages (from-to)129-132
Number of pages4
JournalJapanese Journal of Medical Mycology
Volume46
Issue number2
Publication statusPublished - 2005

Fingerprint

Trichophyton
Paraffin
Polymerase Chain Reaction
Skin
Arthrodermataceae
Hair Follicle
Mycoses
Eosine Yellowish-(YS)
Forearm
Formaldehyde
Fungi
Coloring Agents
DNA
Growth
Genes

Keywords

  • Inflammatory tinea corporis
  • PCR
  • Trichophytia profunda acuta of the glabrous skin
  • Trichophyton rubrum

ASJC Scopus subject areas

  • Microbiology

Cite this

Identification of Trichophyton rubrum by nested PCR analysis from paraffin embedded specimen in trichophytia profunda acuta of the glabrous skin. / Nagao, Keisuke; Sugita, Takashi; Ouchi, Takeshi; Nishikawa, Takeji.

In: Japanese Journal of Medical Mycology, Vol. 46, No. 2, 2005, p. 129-132.

Research output: Contribution to journalArticle

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